TWIST1 Polyclonal antibody

TWIST1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18125-1-AP

Synonyms

ACS3, bHLHa38, BPES2, BPES3, H twist, SCS, TWIST, twist homolog 1 (Drosophila), Twist related protein 1, TWIST1



Tested Applications

Positive WB detected inPC-3 cells, MCF-7 cells, SGC-7901 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18125-1-AP is TWIST1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TWIST1 fusion protein Ag12700
Full Name twist homolog 1 (Drosophila)
Calculated molecular weight 202 aa, 21 kDa
Observed molecular weight 22 kDa, 28 kDa, 45-60 kDa
GenBank accession numberBC036704
Gene symbol TWIST1
Gene ID (NCBI) 7291
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TWIST1, a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. It is also implicated in tumorigenesis, invasion and metastasis. TWIST1 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).

Protocols

Product Specific Protocols
WB protocol for TWIST1 antibody 18125-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC,IF

Theranostics

Twist1 downregulation of PGC-1α decreases fatty acid oxidation in tubular epithelial cells, leading to kidney fibrosis.

Authors - Limin Liu
humanWB

Cancer Lett

Downregulation of ACE2/Ang-(1-7)/Mas axis promotes breast cancer metastasis by enhancing store-operated calcium entry.

Authors - Changhui Yu
humanWB,IHC

Int J Cancer

Down-regulation of KIAA1199/CEMIP by miR-216a suppresses tumor invasion and metastasis in colorectal cancer.

Authors - Dejun Zhang
humanWB

Sci Rep

Correlations between TBL1XR1 and recurrence of colorectal cancer.

Authors - Hongda Liu
mouseWB

Sci Rep

Conditional ablation of TGF-β signaling inhibits tumor progression and invasion in an induced mouse bladder cancer model.

Authors - Yu Liang
humanWB

Sci Rep

STYK1 promotes epithelial-mesenchymal transition and tumor metastasis in human hepatocellular carcinoma through MEK/ERK and PI3K/AKT signaling.

Authors - Zhaowen Wang