MCF7 cells were subjected to SDS PAGE followed by western blot with 18125-1-AP(TWIST1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

PC-3 cells were subjected to SDS PAGE followed by western blot with 18125-1-AP(TWIST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

SGC-7901 cells were subjected to SDS PAGE followed by western blot with 18125-1-AP( TWIST1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

SGC-7901 cells were subjected to SDS PAGE followed by western blot with 18125-1-AP(TWIST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours