|Positive WB detected in||PC-3 cells, MCF-7 cells, SGC-7901 cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Sample-dependent, check data in validation data gallery|
The immunogen of 18125-1-AP is TWIST1 Fusion Protein expressed in E. coli.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TWIST1 fusion protein Ag12700|
|Full Name||twist homolog 1 (Drosophila)|
|Calculated molecular weight||202 aa, 21 kDa|
|Observed molecular weight||22 kDa, 28 kDa, 45-60 kDa|
|GenBank accession number||BC036704|
|Gene ID (NCBI)||7291|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TWIST1, a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. It is also implicated in tumorigenesis, invasion and metastasis. TWIST1 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).
Twist1 downregulation of PGC-1α decreases fatty acid oxidation in tubular epithelial cells, leading to kidney fibrosis.
Downregulation of ACE2/Ang-(1-7)/Mas axis promotes breast cancer metastasis by enhancing store-operated calcium entry.
Int J Cancer
Down-regulation of KIAA1199/CEMIP by miR-216a suppresses tumor invasion and metastasis in colorectal cancer.
Correlations between TBL1XR1 and recurrence of colorectal cancer.
Conditional ablation of TGF-β signaling inhibits tumor progression and invasion in an induced mouse bladder cancer model.
STYK1 promotes epithelial-mesenchymal transition and tumor metastasis in human hepatocellular carcinoma through MEK/ERK and PI3K/AKT signaling.