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- KD/KO Validated
TWIST1-specific Polyclonal antibody
TWIST1-specific Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, CoIP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 25465-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MDA-MB-453s cells, A549 cells, mouse brain tissue, Raji cells |
| Positive IP detected in | MDA-MB-453s cells |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 85 publications below |
| IHC | See 6 publications below |
| IF | See 2 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
| ChIP | See 2 publications below |
Product Information
The immunogen of 25465-1-AP is TWIST1-specific Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TWIST1-specific fusion protein Ag21924 |
| Full Name | twist homolog 1 (Drosophila) |
| Calculated molecular weight | 202 aa, 21 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC036704 |
| Gene symbol | TWIST1 |
| Gene ID (NCBI) | 7291 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Twist-related protein 1 (TWIST1), also named as BHLHA38, is 202 amino acid protein, which contains one bHLH domain. TWIST1 localizes in the nucleus and acts as a transcriptional regulator. TWIST1 inhibits monogenesis by sequestrating E proteins and inhibits tans-activation by MEF2. TWIST1 is expressed in subset of mesodermal cells. The antibody reacts specifically with twist1 protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
| IHC protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
| IF protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
| IP protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab ACOT12-Dependent Alteration of Acetyl-CoA Drives Hepatocellular Carcinoma Metastasis by Epigenetic Induction of Epithelial-Mesenchymal Transition.
| ||
Cancer Cell Int SIRT6/HIF-1α axis promotes papillary thyroid cancer progression by inducing epithelial-mesenchymal transition. | ||
Biochem Cell Biol The long non-coding RNA HOTTIP promotes breast cancer cell migration, invasion, and epithelial-mesenchymal transition via Wnt/β-catenin pathway. | ||
J Cell Physiol Comparison of phenotypic markers and neural differentiation potential of human bone marrow stromal cells from the cranial bone and iliac crest. | ||
Theranostics Oncogenic MSH6-CXCR4-TGFB1 Feedback Loop: A Novel Therapeutic Target of Photothermal Therapy in Glioblastoma Multiforme. | ||
Oncol Lett Effects of SIRT1 silencing on viability, invasion and metastasis of human glioma cell lines. |
