TWIST1-specific Polyclonal antibody

• Featured Product
• KD/KO Validated

TWIST1-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

109

Cat no : 25465-1-AP

Synonyms

ACS3, bHLHa38, BPES2, BPES3, H twist, SCS, TWIST, twist homolog 1 (Drosophila), Twist related protein 1, TWIST1, TWIST1-specific

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Validation Data Gallery

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  WB analysis of MDA-MB-453s using 25465-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25465-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 25465-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 25465-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MDA-MB-453s using 25465-1-AP

  IP Result of anti-TWIST1-specific (IP:25465-1-AP, 4ug; Detection:25465-1-AP 1:500) with MDA-MB-453s cells lysate 920ug.

• 

  IHC staining of human pancreas cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 25465-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MDA-MB-453s cells, Raji cells, A549 cells, mouse brain tissue
• Positive IP detected in: MDA-MB-453s cells
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 6 publications below
• WB: See 104 publications below
• IHC: See 9 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below
• ChIP: See 3 publications below

Product Information

The immunogen of 25465-1-AP is TWIST1-specific Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TWIST1-specific fusion protein Ag21924
• Full Name: twist homolog 1 (Drosophila)
• Calculated molecular weight: 202 aa, 21 kDa
• Observed molecular weight: 30 kDa
• GenBank accession number: BC036704
• Gene symbol: TWIST1
• Gene ID (NCBI): 7291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Twist-related protein 1 (TWIST1), also named as BHLHA38, is 202 amino acid protein, which contains one bHLH domain. TWIST1 localizes in the nucleus and acts as a transcriptional regulator. TWIST1 inhibits monogenesis by sequestrating E proteins and inhibits tans-activation by MEF2. TWIST1 is expressed in subset of mesodermal cells. The antibody reacts specifically with Twist1 protein. The calculated molecular weight of TWIST1 is 21 kDa, but the observed molecular weight is about 30 kDa due to the post-translational modification(PMID: 30733340) .

Publications

Species	Application	Title
human	WB,IHC	Cell Metab; ACOT12-Dependent Alteration of Acetyl-CoA Drives Hepatocellular Carcinoma Metastasis by Epigenetic Induction of Epithelial-Mesenchymal Transition.; Authors - Ming Lu ; KD Validated
human	WB	Cancer Cell Int; SIRT6/HIF-1α axis promotes papillary thyroid cancer progression by inducing epithelial-mesenchymal transition.; Authors - Zhou Yang
human	WB	Biochem Cell Biol; The long non-coding RNA HOTTIP promotes breast cancer cell migration, invasion, and epithelial-mesenchymal transition via Wnt/β-catenin pathway.; Authors - Sijia Han
human	WB	J Cell Physiol; Comparison of phenotypic markers and neural differentiation potential of human bone marrow stromal cells from the cranial bone and iliac crest.; Authors - Yuyuan Ma
human		Theranostics; Oncogenic MSH6-CXCR4-TGFB1 Feedback Loop: A Novel Therapeutic Target of Photothermal Therapy in Glioblastoma Multiforme.; Authors - Yaodong Chen
human	WB	Oncol Lett; Effects of SIRT1 silencing on viability, invasion and metastasis of human glioma cell lines.; Authors - Yu Li

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