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- KD/KO Validated
TWIST1-specific Polyclonal antibody
TWIST1-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, CoIP, ChIP, ELISA
Conjugate
Unconjugated
109
Cat no : 25465-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MDA-MB-453s cells, Raji cells, A549 cells, mouse brain tissue |
Positive IP detected in | MDA-MB-453s cells |
Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 6 publications below |
WB | See 104 publications below |
IHC | See 9 publications below |
IF | See 3 publications below |
IP | See 3 publications below |
CoIP | See 2 publications below |
ChIP | See 3 publications below |
Product Information
The immunogen of 25465-1-AP is TWIST1-specific Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TWIST1-specific fusion protein Ag21924 |
Full Name | twist homolog 1 (Drosophila) |
Calculated molecular weight | 202 aa, 21 kDa |
Observed molecular weight | 30 kDa |
GenBank accession number | BC036704 |
Gene symbol | TWIST1 |
Gene ID (NCBI) | 7291 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Twist-related protein 1 (TWIST1), also named as BHLHA38, is 202 amino acid protein, which contains one bHLH domain. TWIST1 localizes in the nucleus and acts as a transcriptional regulator. TWIST1 inhibits monogenesis by sequestrating E proteins and inhibits tans-activation by MEF2. TWIST1 is expressed in subset of mesodermal cells. The antibody reacts specifically with Twist1 protein. The calculated molecular weight of TWIST1 is 21 kDa, but the observed molecular weight is about 30 kDa due to the post-translational modification(PMID: 30733340) .
Protocols
Product Specific Protocols | |
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WB protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
IHC protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
IF protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
IP protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Metab ACOT12-Dependent Alteration of Acetyl-CoA Drives Hepatocellular Carcinoma Metastasis by Epigenetic Induction of Epithelial-Mesenchymal Transition.
| ||
Cancer Cell Int SIRT6/HIF-1α axis promotes papillary thyroid cancer progression by inducing epithelial-mesenchymal transition. | ||
Biochem Cell Biol The long non-coding RNA HOTTIP promotes breast cancer cell migration, invasion, and epithelial-mesenchymal transition via Wnt/β-catenin pathway. | ||
J Cell Physiol Comparison of phenotypic markers and neural differentiation potential of human bone marrow stromal cells from the cranial bone and iliac crest. | ||
Theranostics Oncogenic MSH6-CXCR4-TGFB1 Feedback Loop: A Novel Therapeutic Target of Photothermal Therapy in Glioblastoma Multiforme. | ||
Oncol Lett Effects of SIRT1 silencing on viability, invasion and metastasis of human glioma cell lines. |