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TWIST1-specific Polyclonal antibody
TWIST1-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 25465-1-AP
Validation Data Gallery
|Positive WB detected in||U-251 cells, mouse brain tissue, Raji cells, SH-SY5Y cells, A549 cells, MDA-MB-231 cells, PC-3 cells|
|Positive IP detected in||MDA-MB-453s cells|
|Positive IHC detected in||human liver cancer tissue, human breast cancer tissue, human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
25465-1-AP targets TWIST1-specific in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TWIST1-specific fusion protein Ag21924|
|Full Name||twist homolog 1 (Drosophila)|
|Calculated molecular weight||202 aa, 21 kDa|
|Observed molecular weight||25-30 kDa|
|GenBank accession number||BC036704|
|Gene ID (NCBI)||7291|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Twist-related protein 1 (TWIST1), also named as BHLHA38, is 202 amino acid protein, which contains one bHLH domain. TWIST1 localizes in the nucleus and acts as a transcriptional regulator. TWIST1 inhibits monogenesis by sequestrating E proteins and inhibits tans-activation by MEF2. TWIST1 is expressed in subset of mesodermal cells. The antibody reacts specifically with Twist1 protein. The calculated molecular weight of TWIST1 is 21 kDa, but the observed molecular weight is about 30 kDa due to the post-translational modification(PMID: 30733340).
|Product Specific Protocols|
|WB protocol for TWIST1-specific antibody 25465-1-AP||Download protocol|
|IHC protocol for TWIST1-specific antibody 25465-1-AP||Download protocol|
|IF protocol for TWIST1-specific antibody 25465-1-AP||Download protocol|
|IP protocol for TWIST1-specific antibody 25465-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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