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TWIST1-specific Polyclonal antibody
TWIST1-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA
Conjugate
Unconjugated
165
Cat no : 25465-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | U-251 cells, Raji cells, A549 cells, SH-SY5Y cells, MDA-MB-231 cells, PC-3 cells |
Positive IP detected in | MDA-MB-453s cells |
Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | L02 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 8 publications below |
WB | See 153 publications below |
IHC | See 20 publications below |
IF | See 8 publications below |
IP | See 5 publications below |
CoIP | See 2 publications below |
ChIP | See 4 publications below |
Product Information
25465-1-AP targets TWIST1-specific in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TWIST1-specific fusion protein Ag21924 |
Full Name | twist homolog 1 (Drosophila) |
Calculated Molecular Weight | 202 aa, 21 kDa |
Observed Molecular Weight | 25-30 kDa |
GenBank Accession Number | BC036704 |
Gene Symbol | TWIST1 |
Gene ID (NCBI) | 7291 |
RRID | AB_2880093 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Twist-related protein 1 (TWIST1), also named as BHLHA38, is 202 amino acid protein, which contains one bHLH domain. TWIST1 localizes in the nucleus and acts as a transcriptional regulator. TWIST1 inhibits monogenesis by sequestrating E proteins and inhibits tans-activation by MEF2. TWIST1 is expressed in subset of mesodermal cells. The antibody reacts specifically with Twist1 protein. The calculated molecular weight of TWIST1 is 21 kDa, but the observed molecular weight is about 30 kDa due to the post-translational modification(PMID: 30733340).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
IHC protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
IF protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
IP protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Adv Phospholipid remodeling is critical for stem cell pluripotency by facilitating mesenchymal-to-epithelial transition. | ||
J Exp Clin Cancer Res ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK | ||
Int J Biol Macromol Fucoidan/chitosan layered PLGA nanoparticles with melatonin loading for inducing intestinal absorption and addressing triple-negative breast cancer progression | ||
Clin Transl Med FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression. | ||
Clin Transl Med Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma. | ||
EMBO Rep Mouse endothelial OTUD1 promotes angiotensin II-induced vascular remodeling by deubiquitinating SMAD3 |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Pramod Bahadur (Verified Customer) (02-27-2024) | We are missing the vials so I dont have lot number.
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