TXK Polyclonal antibody

TXK Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18981-1-AP

Synonyms

PTK4, PSCTK5, Protein-tyrosine kinase 4, Protein tyrosine kinase 4, EC:2.7.10.2

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Validation Data Gallery

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  IHC staining of human lymphoma using 18981-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 18981-1-AP (TXK antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lymphoma using 18981-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 18981-1-AP (TXK antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 18981-1-AP

  Immunofluorescent analysis of Hela cells, using TXK antibody 18981-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 18981-1-AP

  Immunofluorescent analysis of HepG2 cells, using TXK antibody 18981-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells, HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

18981-1-AP targets TXK in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: TXK tyrosine kinase
• Calculated Molecular Weight: 61 kDa
• GenBank Accession Number: NM_003328
• Gene Symbol: TXK
• Gene ID (NCBI): 7294
• RRID: AB_10598482
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXK, also named as PTK4, is an enzyme that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation.

Publications

Species	Application	Title
human	IHC	Biomedicines; Establishing Molecular Subgroups of CD8+ T Cell-Associated Genes in the Ovarian Cancer Tumour Microenvironment and Predicting the Immunotherapy Response; Authors - Yunshu Zhu