Tested Applications
| Positive WB detected in | human heart tissue, mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22090-1-AP targets TXLNB in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17295 Product name: Recombinant human TXLNB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 335-684 aa of BC115383 Sequence: LLNQAAEWKLQAKMLKEQETVLQAQLTLYSGRFEEFQSTLTKSNEVFATFKQEMDKTTKKMKKLEKDTATWKARFENCNKALLDMIEEKALRAKEYECFVMKIGRLENLCRALQEERNELHKKIRDAEISEKDDQSQHNSDEEPESNVSVDQEIDAEEVNSVQTAVKNLATAFMIIHHPESTPHQSKETQPEIGSSQESADAALKEPEQPPLIPSRDSESPLPPLTPQAEAEGGSDAEPPSKASNSPAGLGAETQCEGLPVGAQADQPSWKPEAEASGQAPQAPTEASLQKMEADVPAPACAAEEHVAAMVPACEPSRQPPRAAAEELPVGASAGPQPRNVADTNLEGVD Predict reactive species |
| Full Name | taxilin beta |
| Calculated Molecular Weight | 684 aa, 77 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC115383 |
| Gene Symbol | TXLNB |
| Gene ID (NCBI) | 167838 |
| RRID | AB_3742066 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N3L3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TXLNB (Taxilin beta, gliding protein beta) is a protein enriched predominantly in cardiomyocytes and belongs to the centrosome articulating protein family. It is localized in the perinuclear region of the centrosome and is closely associated with the microtubule organizing center (MTOC) and the ubiquitin-proteasome system (UPS). TXLNB plays an important role in protein homeostasis in cardiomyocytes, affecting intracellular protein degradation and quality control by regulating the interactions between centrosomes and proteasomes. TXLNB was found to have a key role in cardiomyocyte hypertrophy and cardiac proteotoxicity, and its overexpression enhances proteasome activity and reduces the accumulation of ubiquitylated proteins, whereas knockdown leads to proteasome insufficiency and a decline in cardiac function with age. In addition, TXLNB may be involved in the dynamic regulation of the cytoskeleton and microtubules.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TXLNB antibody 22090-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
