ERp19 Polyclonal antibody

ERp19 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20181-1-AP

Synonyms

AGR1, AIF, AIFM1, ERp18, ERp19, hAG 1, PDCD8, PDIA16, TLP19, TXNDC12

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Validation Data Gallery

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  WB analysis of human brain using 20181-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20181-1-AP (ERp19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 20181-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 20181-1-AP (ERp19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 20181-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20181-1-AP (ERp19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20181-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20181-1-AP (ERp19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 20181-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20181-1-AP (ERp19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20181-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20181-1-AP (ERp19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 20181-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20181-1-AP (ERp19 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 20181-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20181-1-AP (ERp19 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 20181-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20181-1-AP (ERp19 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 20181-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20181-1-AP (ERp19 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, human heart tissue, mouse kidney tissue, mouse lung tissue
• Positive IHC detected in: human liver cancer tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20181-1-AP targets ERp19 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERp19 fusion protein Ag14045
• Full Name: thioredoxin domain containing 12 (endoplasmic reticulum)
• Calculated Molecular Weight: 172 aa, 19 kDa
• Observed Molecular Weight: 20 kDa, 40 kDa
• GenBank Accession Number: BC001493
• Gene Symbol: TXNDC12
• Gene ID (NCBI): 51060
• RRID: AB_10646468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXNDC12, which is known as thioredoxin domain-containing protein 12, ERp16, ERp18, ERp19, or hTLP19, is a member of the protein disulfide isomerase (PDI) family that plays important roles in cancer development and progression. TXNDC12 has been demonstrated to contribute to tumorigenicity in human gastric cancer by promoting cell growth, migration, and invasion(PMID: 25940440). Other studies also revealed the overexpression of TXNDC12 promotes HCC metastasis through β-catenin-mediated induction of EMT and that TXNDC12 has the potential to be a prognostic factor and therapeutic target of HCC. (PMID: 31570854)