Tested Applications
| Positive WB detected in | HepG2 cells, human skeletal muscle tissue, COLO 320 cells, mouse colon tissue, human placenta tissue |
| Positive IHC detected in | human stomach cancer tissue, human stomach tissue, human colon cancer tissue, human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19834-1-AP targets TXNDC5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13808 Product name: Recombinant human TXNDC5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-324 aa of BC001199 Sequence: MEDAKVYVAKVDCTAHSDVCSAQGVRGYPTLKLFKPGQEAVKYQGPRDFQTLENWMLQTLNEEPVTPEPEVEPPSAPELKQGLYELSASNFELHVAQGDHFIKFFAPWCGHCKALAPTWEQLALGLEHSETVKIGKVDCTQHYELCSGNQVRGYPTLLWFRDGKKVDQYKGKRDLESLREYVESQLQRTETGATETVTPSEAPVLAAEPEADKGTVLALTENNFDDTIAEGITFIKFYAPWCGHCKTLAPTWEELSKKEFPGLAGVKIAEVDCTAERNICSKYSVRGYPTLLLFRGGKKVSEHSGGRDLDSLHRFVLSQAKDEL Predict reactive species |
| Full Name | thioredoxin domain containing 5 (endoplasmic reticulum) |
| Calculated Molecular Weight | 432 aa, 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC001199 |
| Gene Symbol | TXNDC5 |
| Gene ID (NCBI) | 81567 |
| RRID | AB_10644285 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NBS9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TXNDC5 antibody 19834-1-AP | Download protocol |
| IHC protocol for TXNDC5 antibody 19834-1-AP | Download protocol |
| WB protocol for TXNDC5 antibody 19834-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-TXNDC5 antibody (Cat# 19834-1-AP) has accumulated 33 citations across journals including Nature Communications, Journal of Clinical Investigation, Science Advances, Cancer Research, ACS Nano, Cell Reports, International Journal of Molecular Sciences, Journal of Biological Chemistry, and others. Research fields span fibrosis (pulmonary, renal, testicular), cancer (colorectal, bladder, glioma, melanoma), endoplasmic reticulum stress, cardiovascular disease, platelet biology, lipid metabolism, inflammation, liver disease, spermatogenesis, and viral infection.
| Species | Application | Title |
|---|---|---|
Cancer Res A Genome-Wide Synthetic Lethal Screen Identifies Spermidine Synthase as a Target to Enhance Erdafitinib Efficacy in FGFR-Mutant Bladder Cancer | ||
Nat Commun Fibroblast-enriched endoplasmic reticulum protein TXNDC5 promotes pulmonary fibrosis by augmenting TGFβ signaling through TGFBR1 stabilization.
| ||
Nat Commun Targeting fibroblast TXNDC5 resolves tumor desmoplasia and PD-1 resistance in colorectal cancer with mesenchymal traits. | ||
J Nanobiotechnology Determining M2 macrophages content for the anti-tumor effects of metal-organic framework-encapsulated pazopanib nanoparticles in breast cancer |
