TXNDC9 Polyclonal antibody

• Featured Product
• KD/KO Validated

TXNDC9 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25208-1-AP

Synonyms

APACD, PHLP3, Protein 1 4, TXNDC9

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Validation Data Gallery

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  WB analysis of HeLa using 25208-1-AP

  WB result of TXNDC9 antibody (25208-1-AP; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNDC9 transfected HeLa cells.

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  WB analysis of Raji using 25208-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25208-1-AP (TXNDC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25208-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25208-1-AP (TXNDC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 25208-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25208-1-AP (TXNDC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 25208-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 25208-1-AP (TXNDC9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Raji cells, HEK-293 cells, K-562 cells, HeLa cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25208-1-AP targets TXNDC9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TXNDC9 fusion protein Ag19509
• Full Name: thioredoxin domain containing 9
• Calculated Molecular Weight: 226 aa, 27 kDa
• Observed Molecular Weight: 25-27 kDa
• GenBank Accession Number: BC005968
• Gene Symbol: TXNDC9
• Gene ID (NCBI): 10190
• RRID: AB_2879961
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXNDC9, also known as APACD, has a negative effect on protein folding by significantly reducing the activity of chaperone protein TCP1 complex ATPase activity. Including actin or tubulin.