TXNIP Polyclonal antibody

• Featured Product
• KD/KO Validated

TXNIP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

83

Cat no : 18243-1-AP

Synonyms

Vitamin D3 up-regulated protein 1, VDUP1, Thioredoxin-interacting protein, Thioredoxin-binding protein 2, thioredoxin interacting protein

---

Validation Data Gallery

• 

  WB analysis of K-562 using 18243-1-AP

  WB result of TXNIP antibody (18243-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNIP transfected K-562 cells.

• 

  WB analysis of HL-60 using 18243-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 18243-1-AP (TXNIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18243-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18243-1-AP (TXNIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18243-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18243-1-AP (TXNIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18243-1-AP (TXNIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18243-1-AP (TXNIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18243-1-AP (TXNIP Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18243-1-AP (TXNIP Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse colon using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18243-1-AP (TXNIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18243-1-AP (TXNIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18243-1-AP (TXNIP Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse kidney using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18243-1-AP (TXNIP Antibody) at dilution of 1:200 (under 40x lens).

---

Tested Applications

• Positive WB detected in: HL-60 cells, K-562 cells, MCF-7 cells, PC-12 cells
• Positive IHC detected in: human kidney tissue, human ovary tumor tissue, mouse colon tissue, mouse kidney tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 68 publications below
• IHC: See 22 publications below
• IF: See 13 publications below
• IP: See 3 publications below
• FC: See 1 publications below

Product Information

18243-1-AP targets TXNIP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TXNIP fusion protein Ag13009
• Full Name: thioredoxin interacting protein
• Calculated Molecular Weight: 391 aa, 44 kDa
• Observed Molecular Weight: 44-55 kDa
• GenBank Accession Number: BC093702
• Gene Symbol: TXNIP
• Gene ID (NCBI): 10628
• RRID: AB_2876873
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXNIP, also known as VDUP-1 or TBP-2, belongs to alpha-arrestin protein family and is perhaps the only family member known to bind thioredoxin (TRX). TXNIP was induced by Vitamin D3, but not induced by another monocyte or macrophage differentiation inducer: phorbol 12-myristate 13-acetate (PMA). TXNIP bound catalytic active center of thioredoxin (TRX), which protected cells against oxidative stress. TXNIP was found to be a negative regulator of thioredoxin activity and inducer of the intracellular level of reactive oxygen species (ROS). TXNIP plays an important role in a wide variety of biological functions, such as the regulation of cell death, growth, differentiation, and energy metabolism.

Publications

Species	Application	Title
mouse	FC	Gastroenterology; MondoA-Thioredoxin-Interacting Protein Axis Maintains Regulatory T-Cell Identity and Function in Colorectal Cancer Microenvironment.; Authors - Ying Lu
human	WB	Clin Cancer Res; FBW7 (F-box and WD repeat domain-containing 7) negatively regulates glucose metabolism by targeting the c-Myc/TXNIP (thioredoxin binding protein) axis in pancreatic cancer.; Authors - Shunrong Ji
mouse	IHC	Theranostics; Epigenetic repression of miR-17 contributed to di(2-ethylhexyl) phthalate-triggered insulin resistance by targeting Keap1-Nrf2/miR-200a axis in skeletal muscle.; Authors - Jie Wei
human	WB	Redox Biol; PMID: 32330868; Authors - Yifan Jia ; KD Validated
rat	WB	Oxid Med Cell Longev; PMID: 31998437; Authors - Jing Yu
mouse	WB,IF	Oxid Med Cell Longev; PPARγ Alleviates Sepsis-Induced Liver Injury by Inhibiting Hepatocyte Pyroptosis via Inhibition of the ROS/TXNIP/NLRP3 Signaling Pathway.; Authors - Zeyu Li

Related Products

Thioredoxin Polyclonal antibody

14999-1-AP

FOXO1 Polyclonal antibody

18592-1-AP

MLXIP Polyclonal antibody

13614-1-AP

HIF-1 alpha Polyclonal antibody

20960-1-AP

TXNRD1 Polyclonal antibody

11117-1-AP

SCEL Polyclonal antibody

11906-1-AP

Annexin A1 Polyclonal antibody

55018-1-AP

Annexin A1 Polyclonal antibody

21990-1-AP

HDAC10 Polyclonal antibody

24913-1-AP