TXNL1 Polyclonal antibody

TXNL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15289-1-AP

Synonyms

TRP32, TXL, Thioredoxin-like protein 1, Thioredoxin like protein 1, thioredoxin like 1

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Validation Data Gallery

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  WB analysis of K-562 using 15289-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15289-1-AP (TXNL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 15289-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15289-1-AP (TXNL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 15289-1-AP

  IP result of anti-TXNL1 (IP:15289-1-AP, 4ug; Detection:15289-1-AP 1:300) with HL-60 cells lysate 960ug.

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  IHC staining of human heart using 15289-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15289-1-AP (TXNL1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 15289-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15289-1-AP (TXNL1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human thyroid cancer using 15289-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15289-1-AP (TXNL1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human thyroid cancer using 15289-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15289-1-AP (TXNL1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of RAW 264.7 using 15289-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using 15289-1-AP (TXNL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, HL-60 cells
• Positive IP detected in: HL-60 cells
• Positive IHC detected in: human heart tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

15289-1-AP targets TXNL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TXNL1 fusion protein Ag7481
• Full Name: thioredoxin-like 1
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC001156
• Gene Symbol: TXNL1
• Gene ID (NCBI): 9352
• RRID: AB_2288008
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXNL1(Thioredoxin-like protein 1) is also named as TRP32, TXL, TXNL and belongs to the thioredoxin family. It might be involved in the cellular response to sugar starvation stress. The predicted 289-amino acid, 32.3-kD protein has 2 distinct domains: an N-terminal domain, which is 43% identical to human TXN, and a C-terminal domain, which showed no homology to other proteins in the sequence databases(PMID:9473519).TXNL1 functions as an effector of oxidants or redox sensor that couples oxidative stress to endocytosis, by converting redox changes into a specific GDI:Rab5-mediated endocytic response(PMID:17987124).

Publications

Species	Application	Title
human	WB,IHC	Cell Death Dis; TXNL1-XRCC1 pathway regulates cisplatin-induced cell death and contributes to resistance in human gastric cancer.; Authors - W Xu
human	WB	Curr Cancer Drug Targets; TXNL1 induces apoptosis in cisplatin resistant human gastric cancer cell lines.; Authors - Pan Ni
	WB	Vet Res; Newcastle disease virus V protein inhibits apoptosis in DF-1 cells by downregulating TXNL1.; Authors - Caiying Wang