TXNRD2 Polyclonal antibody, PBS Only

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• TXNRD2 Polyclonal antibody, PBS Only (16360-1-PBS)

• 

  WB analysis using 16360-1-AP (same clone as 16360-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16360-1-AP (TXNRD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16360-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 16360-1-AP (same clone as 16360-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16360-1-AP (TXNRD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16360-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 16360-1-AP (same clone as 16360-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16360-1-AP (TXNRD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16360-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16360-1-AP (same clone as 16360-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16360-1-AP (TXNRD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16360-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16360-1-AP (same clone as 16360-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16360-1-AP (TXNRD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16360-1-PBS in a different storage buffer formulation.

TXNRD2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 16360-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

16360-1-PBS targets TXNRD2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8367

  Product name: Recombinant human TXNRD2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 137-485 aa of BC007489

  Sequence: EILLSADHIIIATGGRPRYPTHIEGALEYGITSDDIFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTIMMRSIPLRGFDQQMSSMVIEHMASHGTRFLRGCAPSRVRRLPDGQLQVTWEDSTTGKEDTGTFDTVLWAIGRVPDTRSLNLEKAGVDTSPDTQKILVDSREATSVPHIYAIGDVVEGRPELTPIAIMAGRLLVQRLFGGSSDLMDYDNVPTTVFTPLEYGCVGLSEEEAVARHGQEHVEVYHAHYKPLEFTVAGRDASQCYVKMVCLREPPQLVLGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTVGIHPTCSEEVVKLRISKRSGLDPTVTGCUG

• Full Name: thioredoxin reductase 2
• Calculated Molecular Weight: 524 aa, 56 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC007489
• Gene Symbol: TXNRD2
• Gene ID (NCBI): 10587
• RRID: AB_2210258
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NNW7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Human mitochondrial thioredoxin reductase (TXNRD2) is a selenocysteine-containing enzyme essential for mitochondrial oxygen radical scavenging. Three mammalian thioredoxin reductases exist; a cytosolic (TXNRD1), a mitochondrial (TXNRD2), and a testis-specific thioredoxin reductase (TXNRD3). (PMID: 21247928, PMID: 26199228)