TXNRD3 Polyclonal antibody

TXNRD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 19517-1-AP

Synonyms

TGR, thioredoxin reductase 3, Thioredoxin reductase TR2, TR2, TRXR3, TXNRD3

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Validation Data Gallery

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  WB analysis using 19517-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19517-1-AP (TXNRD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 19517-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19517-1-AP (TXNRD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 19517-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19517-1-AP (TXNRD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 19517-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19517-1-AP (TXNRD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue, human testis tissue, rat testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

19517-1-AP targets TXNRD3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: thioredoxin reductase 3
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: XM_001130163
• Gene Symbol: TXNRD3
• Gene ID (NCBI): 114112
• RRID: AB_10644343
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXNRD3, also named as TGR and TRXR3, belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. TXNRD3 displays thioredoxin reductase, glutaredoxin and glutathione reductase activities. TXNRD3 catalyzes the reaction: Thioredoxin + NADP+ = thioredoxin disulfide + NADPH. TXNRD3 promotes disulfide bond formation between GPX4 and various sperm proteins and may play a role in sperm maturation by promoting formation of sperm structural components.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer; Authors - Rong Wang
human	WB	Nat Chem Biol; Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability.; Authors - Zhipeng Li
pig	WB	Front Vet Sci; The Selenium Yeast vs Selenium Methionine on Cell Viability, Selenoprotein Profile and Redox Status via JNK/ P38 Pathway in Porcine Mammary Epithelial Cells.; Authors - Caichi Wu
mouse	WB,IF	Life Sci; Supression Thioredoxin reductase 3 exacerbates the progression of liver cirrhosis via activation of ferroptosis pathway; Authors - Qi Liu