TYW1B Polyclonal antibody

TYW1B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17665-1-AP

Synonyms

NCRNA00069, RSAFD2, TYW1B

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Validation Data Gallery

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  WB analysis of mouse liver using 17665-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17665-1-AP (TYW1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17665-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17665-1-AP (TYW1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary using 17665-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17665-1-AP (TYW1B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 17665-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17665-1-AP (TYW1B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 17665-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17665-1-AP (TYW1B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 17665-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17665-1-AP (TYW1B antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, MCF-7 cells
• Positive IHC detected in: human ovary tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17665-1-AP targets TYW1B in WB, IHC, ELISA applications and shows reactivity with mouse, human samples.

• Tested Reactivity: mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TYW1B fusion protein Ag11947
• Full Name: tRNA-yW synthesizing protein 1 homolog B (non-protein coding)
• Calculated Molecular Weight: 668 aa, 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC068520
• Gene Symbol: TYW1B
• Gene ID (NCBI): 441250
• RRID: AB_2211307
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.