TYW3 Polyclonal antibody

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  WB analysis using 29213-1-AP

  U-251 cell lysates were subjected to SDS PAGE followed by western blot with 29213-1-AP (TYW3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 29213-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TYW3 antibody (29213-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

TYW3 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 29213-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

C1orf171, FLJ40918, tRNA yW synthesizing protein 3

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Tested Applications

• Positive WB detected in: U-251 cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29213-1-AP targets TYW3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30156

  Product name: Recombinant human TYW3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-259 aa of NM_138467

  Sequence: MDRSAEFRKWKAQCLSKADLSRKGSVDEDVVELVQFLNMRDQFFTTSSCAGRILLLDRGINGFEVQKQNCCWLLVTHKLCVKDDVIVALKKANGDATLKFEPFVLHVQCRQLQDAQILHSMAIDSGFRNSGITVGKRGKTMLAVRSTHGLEVPLSHKGKLMVTEEYIDFLLNVANQKMEENKKRIERFYNCLQHALERETMTNLHPKIKEKNNSSYIHKKKRNPEKTRAQCITKESDEELENDDDDDLGINVTIFPEDY

• Full Name: tRNA-yW synthesizing protein 3 homolog (S. cerevisiae)
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: NM_138467
• Gene Symbol: TYW3
• Gene ID (NCBI): 127253
• RRID: AB_2918250
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6IPR3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.