Thrombopoietin Polyclonal antibody

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  WB analysis using 31259-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31259-1-AP (Thrombopoietin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Thrombopoietin Polyclonal Antibody for WB, ELISA

Cat No. 31259-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

Thpo, TPO, Mpllg

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31259-1-AP targets Thrombopoietin in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: thrombopoietin
• Calculated Molecular Weight: 38KD
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: NM_001173505.1
• Gene Symbol: Thrombopoietin
• Gene ID (NCBI): 21832
• RRID: AB_3669921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P40226
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thrombopoietin (TPO) is a primary regulator of megakaryocyte development and platelet production in mammals. Human TPO constitutively circulates and maintains thrombopoiesis through interaction with its cognate receptor, myeloproliferative leukemia protein(MPL). TPO also plays an important role in the maintenance and regulation of hematopoietic stem cells (HSCs).