Thrombopoietin Monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP50053-1

  Sandwich ELISA standard curve of MP50053-1, THPO Monoclonal Matched Antibody Pair, PBS Only. 68672-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  HZ-1248. 68672-2-PBS was HRP conjugated as detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP50053-1

  HL-60 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human Thrombopoietin, and measured 53.6 pg/mL.

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  Sample test of MP50053-1

  Serum of sixteen individual healthy human donors was measured. The human Thrombopoietin concentration of detected samples was determined to be 100.9 pg/mL with a range of ND - 191.1 pg/mL.

Thrombopoietin Monoclonal Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 68672-2-PBS

Clone No.1A8G2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

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Product Information

68672-2-PBS targets Thrombopoietin as part of a matched antibody pair:

MP50053-1: 68672-1-PBS capture and 68672-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Affinity:
  K_D=1.85 x 10^-9M
  K_Off=3.08 x 10^-4M
  K_On=1.67 x 10^5M
• Immunogen: Thrombopoietin fusion protein HZ-1248
• Full Name: thrombopoietin
• Gene Symbol: Thrombopoietin
• Gene ID (NCBI): 7066
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P40225
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.