Tested Applications
| Positive FC detected in | HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.15 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98462-3-RR targets Tie-2/CD202b in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Tie-2/CD202b fusion protein Eg3367 Predict reactive species |
| Full Name | TEK tyrosine kinase, endothelial |
| Calculated Molecular Weight | 1124 aa, 126 kDa |
| GenBank Accession Number | BC035514 |
| Gene Symbol | Tie2 |
| Gene ID (NCBI) | 7010 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q02763 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
Tie-2, also known as CD202b or TEK, is a receptor tyrosine kinase (RTK) specifically expressed in endothelial cells. It plays a crucial role in angiogenesis, forming new blood vessels, and maintaining vascular stability. Tie-2 is a member of the Tie receptor family, characterized by the presence of immunoglobulin and epidermal growth factor (EGF) homology domains. Tie-2 plays a significant role in several vascular diseases, including vascular malformations like venous malformations. It is also implicated in pathological vascular remodeling associated with tumor growth, where it is often overexpressed in tumor vessels.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Tie-2/CD202b antibody 98462-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
