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Timp-3 Polyclonal antibody

Timp-3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10858-1-AP

Synonyms

HSMRK222, K222, K222TA2, Metalloproteinase inhibitor 3, Protein MIG 5, SFD, Timp 3, TIMP3, Timp-3



Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman stomach tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

10858-1-AP targets Timp-3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Timp-3 fusion protein Ag1253
Full Name TIMP metallopeptidase inhibitor 3
Calculated molecular weight 24 kDa
Observed molecular weight 21-24 kDa
GenBank accession numberBC014277
Gene symbol TIMP3
Gene ID (NCBI) 7078
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Timp-3 antibody 10858-1-APDownload protocol
IHC protocol for Timp-3 antibody 10858-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Theranostics

miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury.

Authors - Jing Shi
mouseIHC

Sci Rep

AMPK deficiency in chondrocytes accelerated the progression of instability-induced and ageing-associated osteoarthritis in adult mice.

Authors - Sheng Zhou
ratIHC

Cell Prolif

Aspirin inhibits inflammation and scar formation in the injury tendon healing through regulating JNK/STAT-3 signalling pathway.

Authors - Yunjiao Wang
mouseWB

Biomolecules

Tau Protein Modulates Perineuronal Extracellular Matrix Expression in the TauP301L-acan Mouse Model.

Authors - Sophie Schmidt
mouseIHC

Lab Invest

Timp-3 deficiency impairs cognitive function in mice.

Authors - Baba Yoshichika Y
humanIHC

PLoS One

Elevated MiR-222-3p Promotes Proliferation and Invasion of Endometrial Carcinoma via Targeting ERα.

Authors - Binya Liu