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  • KD/KO Validated

Timp-3 Polyclonal antibody

Timp-3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10858-1-AP

Synonyms

HSMRK222, K222, K222TA2, Metalloproteinase inhibitor 3, Protein MIG 5, SFD, Timp 3, TIMP3



Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman stomach tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

10858-1-AP targets Timp-3 in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Timp-3 fusion protein Ag1253
Full Name TIMP metallopeptidase inhibitor 3
Calculated molecular weight 24 kDa
Observed molecular weight 21-24 kDa
GenBank accession numberBC014277
Gene symbol TIMP3
Gene ID (NCBI) 7078
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Timp-3 antibody 10858-1-APDownload protocol
IHC protocol for Timp-3 antibody 10858-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Onco Targets Ther

miR-222-3p promotes osteosarcoma cell migration and invasion through targeting TIMP3.

Authors - Jianping Guo
IHC

J Toxicol Pathol

Databases for technical aspects of immunohistochemistry.

Authors - Satoshi Furukawa
humanIHC

Med Oncol

EZH2 regulates cancer cell migration through repressing TIMP-3 in non-small cell lung cancer.

Authors - Xu Chunhua C
ratWB

Mol Cell Biochem

Involvement of calcium-sensing receptors in hypoxia-induced vascular remodeling and pulmonary hypertension by promoting phenotypic modulation of small pulmonary arteries.

Authors - Xue Peng
ratWB

Cardiovasc Diagn Ther

MicroRNA-221 promotes proliferation and migration of pulmonary arterial smooth muscle cells (PASMCs) by targeting tissue inhibitor of metalloproteinases-3 (TIMP3).

Authors - Yan Yan
  • KD Validated
humanIHC

PLoS One

Lack of Correlation between Aberrant p16, RAR-β2, TIMP3, ERCC1, and BRCA1 Protein Expression and Promoter Methylation in Squamous Cell Carcinoma Accompanying Candida albicans-Induced Inflammation.

Authors - Yui Terayama