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- KD/KO Validated
Timp-3 Polyclonal antibody
Timp-3 Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC,ELISA
Conjugate
Unconjugated
Cat no : 10858-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human placenta tissue |
| Positive IHC detected in | human stomach tissue, mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 7 publications below |
Product Information
10858-1-AP targets Timp-3 in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Timp-3 fusion protein Ag1253 |
| Full Name | TIMP metallopeptidase inhibitor 3 |
| Calculated molecular weight | 24 kDa |
| Observed molecular weight | 21-24 kDa |
| GenBank accession number | BC014277 |
| Gene symbol | TIMP3 |
| Gene ID (NCBI) | 7078 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Timp-3 antibody 10858-1-AP | Download protocol |
| IHC protocol for Timp-3 antibody 10858-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Onco Targets Ther miR-222-3p promotes osteosarcoma cell migration and invasion through targeting TIMP3. | ||
Med Oncol EZH2 regulates cancer cell migration through repressing TIMP-3 in non-small cell lung cancer. | ||
Mol Cell Biochem Involvement of calcium-sensing receptors in hypoxia-induced vascular remodeling and pulmonary hypertension by promoting phenotypic modulation of small pulmonary arteries. | ||
Cardiovasc Diagn Ther MicroRNA-221 promotes proliferation and migration of pulmonary arterial smooth muscle cells (PASMCs) by targeting tissue inhibitor of metalloproteinases-3 (TIMP3).
| ||
PLoS One Lack of Correlation between Aberrant p16, RAR-β2, TIMP3, ERCC1, and BRCA1 Protein Expression and Promoter Methylation in Squamous Cell Carcinoma Accompanying Candida albicans-Induced Inflammation. |
