Tissue Factor Polyclonal antibody

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  WB analysis using 31768-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31768-1-AP (Tissue Factor antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tissue Factor Polyclonal Antibody for WB, ELISA

Cat No. 31768-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31768-1-AP targets Tissue Factor in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: coagulation factor III
• Calculated Molecular Weight: 33kDa
• Observed Molecular Weight: 45-53 kDa
• Gene Symbol: Tissue Factor
• Gene ID (NCBI): 14066
• RRID: AB_3670107
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P20352
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108). This antibody is specific for mouse Tissue Factor.