Tissue Factor Polyclonal antibody

Tissue Factor Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28005-1-AP

Synonyms

CD142, Coagulation factor III, F3, TF, TFA, Thromboplastin, Tissue factor



Tested Applications

Positive WB detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28005-1-AP targets Tissue Factor in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Tissue Factor fusion protein Ag27553
Full Name coagulation factor III (thromboplastin, tissue factor)
Calculated Molecular Weight 295 aa, 33 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC011029
Gene Symbol F3
Gene ID (NCBI) 2152
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108).

Protocols

Product Specific Protocols
WB protocol for Tissue Factor antibody 28005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Acta Pharmacol Sin

Lethal pulmonary thromboembolism in mice induced by intravenous human umbilical cord mesenchymal stem cell-derived large extracellular vesicles in a dose- and tissue factor-dependent manner

Authors - Bian-Lei Yang