Prealbumin/transthyretin Recombinant antibody, PBS Only (Detector)

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  WB analysis using 86273-1-RR (same clone as 86273-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86273-1-RR (Transthyretin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86273-1-PBS in a different storage buffer formulation.

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  Sample test of MP02304-1

  The mean Prealbumin/TTR concentration was determined to be 172.8 ug/mL in human serum.

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  Cytometric bead array standard curve of MP02304-1

  Cytometric bead array standard curve of MP02304-1, Prealbumin/TTR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86273-2-PBS. Detection antibody: 86273-1-PBS. Standard: Eg0931. Range: 1.56-50 ng/mL.

Prealbumin/transthyretin Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA, Sample test

Cat No. 86273-1-PBS

Clone No.250820B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA, Sample test

Synonyms

Prealbumin, transthyretin, TTR, ATTR, HsT2651

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Product Information

86273-1-PBS targets Prealbumin/transthyretin as part of a matched antibody pair:

MP02304-1: 86273-2-PBS capture and 86273-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Prealbumin/transthyretin fusion protein Eg0931
• Full Name: transthyretin
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: NM_000371.4
• Gene Symbol: TTR
• Gene ID (NCBI): 7276
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02766
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Transthyretin (TTR) is a plasma transport protein for thyroxine and retinol, through the association with retinol-binding protein. It is a homotetrameric protein synthesized mainly in liver, choroid plexus, retinal pigment epithelium, and pancreas.Within the CNS, TTR is the only known protein synthesized solely by the choroid plexus. Mutant and wildtype TTR give rise to various forms of amyloid deposition (amyloidosis). Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR),hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) and carpal tunnel syndrome type 1 (CTS1). In addition, positive immunostaining for TTR has been reported as a sensitive diagnostic marker of choroid plexus tumors. However Western blot analysis using specific rabbit anti-mouse TTR reveals that TTR is absent from the cortex, hippocampus, and cerebellum and present in the choroid plexus. (PMID: 22103483, 16698124)