TrkB Polyclonal antibody

TrkB Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29961-1-AP

Synonyms

GP145 TrkB, NTRK2, Trk B, TRKB, TrkB tyrosine kinase

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Validation Data Gallery

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  WB analysis using 29961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29961-1-AP (NTRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29961-1-AP (TrkB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 29961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29961-1-AP (TrkB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 29961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29961-1-AP (TrkB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 29961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29961-1-AP (NTRK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 29961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29961-1-AP (NTRK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 29961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29961-1-AP (NTRK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 29961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29961-1-AP (NTRK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 29961-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TrkB antibody (29961-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue, mouse cerebellum tissue
• Positive IHC detected in: mouse cerebellum tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29961-1-AP targets TrkB in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TrkB fusion protein Ag32196
• Full Name: neurotrophic tyrosine kinase, receptor, type 2
• Calculated Molecular Weight: 822 aa, 92 kDa
• Observed Molecular Weight: 90-95 kDa, 140-145 kDa
• GenBank Accession Number: BC031835
• Gene Symbol: NTRK2
• Gene ID (NCBI): 4915
• RRID: AB_2918354
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance through regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 238-430 aa of human TrkB recognizes full length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).