TrkB Recombinant monoclonal antibody, PBS Only

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  WB analysis using 80878-6-RR (same clone as 80878-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80878-6-RR (TrkB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80878-6-PBS in a different storage buffer formulation.

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  IP experiment of mouse cerebellum using 80878-6-RR (same clone as 80878-6-PBS)

  IP result of anti-TrkB (IP:80878-6-RR, 4ug; Detection:80878-6-RR 1:4000) with mouse cerebellum tissue lysate 2000 ug. This data was developed using the same antibody clone with 80878-6-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 80878-6-RR (same clone as 80878-6-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80878-6-RR (TrkB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80878-6-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 80878-6-RR (same clone as 80878-6-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80878-6-RR (TrkB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80878-6-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 80878-6-RR (same clone as 80878-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TrkB antibody (80878-6-RR, Clone: 241473G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80878-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80878-6-RR

  Biolayer interferometry (BLl) kinetic assays of 80878-6-RR against Human TrkB were performed. The affinity constant is below 1 pM.

TrkB Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Cat No. 80878-6-PBS

Clone No.241473G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

NTRK2, 241473G5, BDNF/NT-3 growth factors receptor, GP145 TrkB, GP145-TrkB

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Product Information

80878-6-PBS targets TrkB in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3790

  Product name: Recombinant human NTRK2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 41-433 aa of BC031835

  Sequence: SRIWCSDPSPGIVAFPRLEPNSVDPENITEIFIANQKRLEIINEDDVEAYVGLRNLTIVDSGLKFVAHKAFLKNSNLQHINFTRNKLTSLSRKHFRHLDLSELILVGNPFTCSCDIMWIKTLQEAKSSPDTQDLYCLNESSKNIPLANLQIPNCGLPSANLAAPNLTVEEGKSITLSCSVAGDPVPNMYWDVGNLVSKHMNETSHTQGSLRITNISSDDSGKQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHHWCIPFTVKGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTHMNNGDYTLIAKNEYGKDEKQISAHFMGWPGIDDGANPNYPDVIYEDYGTAANDIGDTTNRSNEIPSTDVTDKTGREHLSV

• Full Name: neurotrophic tyrosine kinase, receptor, type 2
• Calculated Molecular Weight: 822 aa, 92 kDa
• Observed Molecular Weight: 90-95 kDa
• GenBank Accession Number: BC031835
• Gene Symbol: TrkB
• Gene ID (NCBI): 4915
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q16620
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain, and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance by regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 41-433aa of human TrkB recognizes the full-length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).