Tyrosinase Polyclonal antibody

• 

  WB analysis using 21995-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21995-1-AP (Tyrosinase antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Tyrosinase Polyclonal Antibody for WB, ELISA

Cat No. 21995-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

Rat, Mouse and More (2)

Applications

WB, IF, ELISA

Synonyms

LB24 AB, Monophenol monooxygenase, OCA1A, OCAIA, SHEP3, SK29 AB, Tumor rejection antigen AB, TYR, Tyrosinase

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 4 publications below

Product Information

21995-1-AP targets Tyrosinase in WB, IF, ELISA applications and shows reactivity with Rat, Mouse samples.

• Tested Reactivity: Rat, Mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17314

  Product name: Recombinant human Tyrosinase protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 19-345 aa of BC027179

  Sequence: HFPRACVSSKNLMEKECCPPWSGDRSPCGQLSGRGSCQNILLSNAPLGPQFPFTGVDDRESWPSVFYNRTCQCSGNFMGFNCGNCKFGFWGPNCTERRLLVRRNIFDLSAPEKDKFFAYLTLAKHTISSDYVIPIGTYGQMKNGSTPMFNDINIYDLFVWMHYYVSMDALLGGSEIWRDIDFAHEAPAFLPWHRLFLLRWEQEIQKLTGDENFTIPYWDWRDAEKCDICTDEYMGGQHPTNPNLLSPASFFSSWQIVCSRLEEYNSHQSLCNGTPEGPLRRNPGNHDKSRTPRLPSSADVEFCLSLTQYESGSMDKAANFSFRNTLE

• Full Name: tyrosinase (oculocutaneous albinism IA)
• Calculated Molecular Weight: 529 aa, 60 kDa
• Observed Molecular Weight: 60 kDa, 70 kDa
• GenBank Accession Number: BC027179
• Gene Symbol: TYR
• Gene ID (NCBI): 7299
• RRID: AB_2918076
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P14679
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF,WB	Acta Pharm Sin B; EHMT2 promotes tumorigenesis in GNAQ/11-mutant uveal melanoma via ARHGAP29-mediated RhoA pathway; Authors - Yongyun Li
human	WB,IF	Gene; The degradation of TYR variants derived from Chinese OCA families is mediated by the ERAD and ERLAD pathway; Authors - Xinyao Wang
mouse	WB,IF	Arch Dermatol Res; N-tert-Butylhydroxylamine promotes melanin production in oxidative stress conditions through the MITF signaling pathway; Authors - Gyeong Hee Kim
mouse	WB,IF	J Cell Biol; LYSMD proteins promote activation of Rab32-family GTPases for lysosome-related organelle biogenesis; Authors - Jinglin Li