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U2AF65 Polyclonal antibody

U2AF65 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 15624-1-AP


hU2AF(65), hU2AF65, U2AF2, U2AF65

Tested Applications

Positive WB detected inJurkat cells, HEK-293T cells, HepG2 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15624-1-AP targets U2AF65 in WB, RIP, IHC, IF applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity bovine, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen U2AF65 fusion protein Ag8070
Full Name U2 small nuclear RNA auxiliary factor 2
Calculated molecular weight 54 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC008740
Gene symbol U2AF2
Gene ID (NCBI) 11338
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

U2AF2, also named as U2 auxiliary factor 65 kDa subunit , is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA, may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.


Product Specific Protocols
WB protocol for U2AF65 antibody 15624-1-APDownload protocol
IHC protocol for U2AF65 antibody 15624-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Biochem Funct

U2AF65 enhances milk synthesis and growth of bovine mammary epithelial cells by positively regulating the mTOR-SREBP-1c signalling pathway.

Authors - Yanbo Yu

J Immunol Res

Circular RNA FOXP1 Induced by ZNF263 Upregulates U2AF2 Expression to Accelerate Renal Cell Carcinoma Tumorigenesis and Warburg Effect through Sponging miR-423-5p.

Authors - Li Fang

Cell Death Dis

RNA binding protein NKAP protects glioblastoma cells from ferroptosis by promoting SLC7A11 mRNA splicing in an m6A-dependent manner.

Authors - Shicheng Sun

Sci Rep

Identification of novel PANDAR protein interaction partners involved in splicing regulation.

Authors - N Pospiech

J Clin Invest

Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.

Authors - Xue Gong


In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Authors - Lei Sun