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U2AF65 Antibody

U2AF65 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15624-1-AP

Synonyms

hU2AF(65), hU2AF65, U2AF2, U2AF65



Tested Applications

Positive WB detected inJurkat cells, HEK-293T cells, HepG2 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15624-1-AP targets U2AF65 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity bovine, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen U2AF65 fusion protein Ag8070
Full Name U2 small nuclear RNA auxiliary factor 2
Calculated molecular weight 54 kDa
Observed molecular weight 60-65 kDa
GenBank accession number BC008740
Gene symbol U2AF2
Gene ID (NCBI) 11338
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

U2AF2, also named as U2 auxiliary factor 65 kDa subunit , is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA, may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.

Protocols

Product Specific Protocols
WB protocol for U2AF65 antibody 15624-1-APDownload protocol
IHC protocol for U2AF65 antibody 15624-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
bovineWB,IF

Cell Biochem Funct

U2AF65 enhances milk synthesis and growth of bovine mammary epithelial cells by positively regulating the mTOR-SREBP-1c signalling pathway.

Authors - Yanbo Yu
humanWB

Sci Rep

Identification of novel PANDAR protein interaction partners involved in splicing regulation.

Authors - N Pospiech