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U2AF65 Polyclonal antibody

U2AF65 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, RIP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15624-1-AP

Synonyms

hU2AF(65), hU2AF65, U2AF2, U2AF65



Tested Applications

Positive WB detected inJurkat cells, HEK-293T cells, HepG2 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15624-1-AP targets U2AF65 in WB, RIP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen U2AF65 fusion protein Ag8070
Full Name U2 small nuclear RNA auxiliary factor 2
Calculated molecular weight 54 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC008740
Gene symbol U2AF2
Gene ID (NCBI) 11338
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

U2AF2, also named as U2 auxiliary factor 65 kDa subunit , is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA, may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.

Protocols

Product Specific Protocols
WB protocol for U2AF65 antibody 15624-1-APDownload protocol
IHC protocol for U2AF65 antibody 15624-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Authors - Lei Sun
mouseIF,RIP

J Clin Invest

Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.

Authors - Xue Gong
humanWB

Sci Rep

Identification of novel PANDAR protein interaction partners involved in splicing regulation.

Authors - N Pospiech
humanWB

iScience

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Authors - Mio Iwasaki
humanWB

Cell Death Dis

RNA binding protein NKAP protects glioblastoma cells from ferroptosis by promoting SLC7A11 mRNA splicing in an m6A-dependent manner.

Authors - Shicheng Sun
humanWB

J Immunol Res

Circular RNA FOXP1 Induced by ZNF263 Upregulates U2AF2 Expression to Accelerate Renal Cell Carcinoma Tumorigenesis and Warburg Effect through Sponging miR-423-5p.

Authors - Li Fang