U2AF65 Polyclonal antibody

• Featured Product
• KD/KO Validated

U2AF65 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, RIP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 15624-1-AP

Synonyms

hU2AF(65), hU2AF65, U2AF2, U2AF65

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Validation Data Gallery

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  WB analysis of HepG2 using 15624-1-AP

  WB result of U2AF65 antibody (15624-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-U2AF65 transfected HepG2 cells.

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  WB analysis of Jurkat using 15624-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 15624-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 15624-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15624-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15624-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 15624-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15624-1-AP (U2AF65 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15624-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15624-1-AP (U2AF65 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293T cells, HepG2 cells, HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Product Information

15624-1-AP targets U2AF65 in WB, RIP, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: U2AF65 fusion protein Ag8070
• Full Name: U2 small nuclear RNA auxiliary factor 2
• Calculated molecular weight: 54 kDa
• Observed molecular weight: 60-65 kDa
• GenBank accession number: BC008740
• Gene symbol: U2AF2
• Gene ID (NCBI): 11338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

U2AF2, also named as U2 auxiliary factor 65 kDa subunit , is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA, may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.

Publications

Species	Application	Title
human	WB	Cell; In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.; Authors - Lei Sun
mouse	IF,RIP	J Clin Invest; Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.; Authors - Xue Gong
human	WB	Sci Rep; Identification of novel PANDAR protein interaction partners involved in splicing regulation.; Authors - N Pospiech
human	WB	iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki
human	WB	Cell Death Dis; RNA binding protein NKAP protects glioblastoma cells from ferroptosis by promoting SLC7A11 mRNA splicing in an m6A-dependent manner.; Authors - Shicheng Sun
human	WB	J Immunol Res; Circular RNA FOXP1 Induced by ZNF263 Upregulates U2AF2 Expression to Accelerate Renal Cell Carcinoma Tumorigenesis and Warburg Effect through Sponging miR-423-5p.; Authors - Li Fang