Validation Data Gallery
|Positive WB detected in||Jurkat cells, HEK-293T cells, HepG2 cells, HeLa cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15624-1-AP targets U2AF65 in WB, RIP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||U2AF65 fusion protein Ag8070|
|Full Name||U2 small nuclear RNA auxiliary factor 2|
|Calculated molecular weight||54 kDa|
|Observed molecular weight||60-65 kDa|
|GenBank accession number||BC008740|
|Gene ID (NCBI)||11338|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
U2AF2, also named as U2 auxiliary factor 65 kDa subunit , is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA, may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.
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