Validation Data Gallery
|Positive WB detected in||Jurkat cells, HEK-293T cells, HepG2 cells, HeLa cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15624-1-AP targets U2AF65 in WB, RIP, IHC, IF applications and shows reactivity with human samples.
|Cited Reactivity||bovine, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||U2AF65 fusion protein Ag8070|
|Full Name||U2 small nuclear RNA auxiliary factor 2|
|Calculated molecular weight||54 kDa|
|Observed molecular weight||60-65 kDa|
|GenBank accession number||BC008740|
|Gene ID (NCBI)||11338|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
U2AF2, also named as U2 auxiliary factor 65 kDa subunit , is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA, may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.
Cell Biochem Funct
U2AF65 enhances milk synthesis and growth of bovine mammary epithelial cells by positively regulating the mTOR-SREBP-1c signalling pathway.
J Immunol Res
Circular RNA FOXP1 Induced by ZNF263 Upregulates U2AF2 Expression to Accelerate Renal Cell Carcinoma Tumorigenesis and Warburg Effect through Sponging miR-423-5p.
Cell Death Dis
RNA binding protein NKAP protects glioblastoma cells from ferroptosis by promoting SLC7A11 mRNA splicing in an m6A-dependent manner.
Identification of novel PANDAR protein interaction partners involved in splicing regulation.
J Clin Invest
Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.
In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.