U2AF35 Monoclonal antibody, PBS Only (Detector)

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  WB analysis using 60289-1-Ig (same clone as 60289-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60289-1-Ig (U2AF35 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60289-1-Ig (same clone as 60289-1-PBS)

  WB result of U2AF35 antibody (60289-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-U2AF35 transfected HeLa cells. This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 60289-1-Ig (same clone as 60289-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60289-1-Ig (U2AF35 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 60289-1-Ig (same clone as 60289-1-PBS)

  IP result of anti-U2AF35 (IP:60289-1-Ig, 3ug; Detection:60289-1-Ig 1:500) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60289-1-Ig (same clone as 60289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60289-1-Ig (U2AF35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60289-1-Ig (same clone as 60289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60289-1-Ig (U2AF35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60289-1-Ig (same clone as 60289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60289-1-Ig (U2AF35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60289-1-Ig (same clone as 60289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60289-1-Ig (U2AF35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 60289-1-Ig (same clone as 60289-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF35 antibody (60289-1-Ig, Clone: 2B7F11 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60289-1-Ig (same clone as 60289-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using U2AF35 antibody (60289-1-Ig, Clone: 2B7F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  FC experiment of Ramos using 60289-1-Ig (same clone as 60289-1-PBS)

  1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human U2AF35 (60289-1-Ig, Clone:2B7F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51496-1

  Cytometric bead array sample test of MP51496-1, U2AF35 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60289-2-PBS. Detection antibody: 60289-1-PBS.

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  Cytometric bead array standard curve of MP51496-1

  Cytometric bead array standard curve of MP51496-1, U2AF35 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60289-2-PBS. Detection antibody: 60289-1-PBS. Standard:Ag0399. Range: 3.125-400 ng/mL.

U2AF35 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Cat No. 60289-1-PBS

Clone No.2B7F11

Host / Isotype

Mouse / IgG2c

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Synonyms

U2AF1, 2B7F11, FP793, RN, RNU2AF1

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Product Information

60289-1-PBS targets U2AF35 as part of a matched antibody pair:

MP51496-1: 60289-2-PBS capture and 60289-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2c
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0399

  Product name: Recombinant human U2AF35 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 26-240 aa of BC001923

  Sequence: ACRHGDRCSRLHNKPTFSQTIALLNIYRNPQNSSQSADGLRCAVSDVEMQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVKFRREEDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGGFCNFMHLKPISRELRRELYGRRRKKHRSRSRSRERRSRSRDRGRGGGGGGGGGGGGRERDRRRSRDRERSGRF

• Full Name: U2 small nuclear RNA auxiliary factor 1
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC001923
• Gene Symbol: U2AF1
• Gene ID (NCBI): 7307
• RRID: AB_2858277
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q01081
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

U2 auxiliary factor (U2AF), comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2 (RNU2) small nuclear RNA auxiliary factor 1 (U2AF1,synonyms: RN, FP793, U2AF35, U2AFBP, RNU2AF1)is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. The U2AF1 gene is localized to chromosome 21q22.3, which is the critical region for three diseases, progressive myoclonus epilepsy, autoimmune polyglandular disease type 1, and one form of bipolar affective disorder. The calculated molecular weight of U2AF35 is 28 kDa, but the modified U2AF35 protein is about 35-40 kDa.