UACA Polyclonal antibody

UACA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 25654-1-AP

Synonyms

KIAA1561, NUCLING, UACA

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Validation Data Gallery

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  WB analysis of HeLa using 25654-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 25654-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 25654-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 25654-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 25654-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 25654-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 25654-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 25654-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 25654-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 25654-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 25654-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A375 using 25654-1-AP

  Immunofluorescent analysis of A375 cells using 25654-1-AP (UACA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A375 cells, HEK-293 cells, Neuro-2a cells, Y79 cells
• Positive IHC detected in: human colon tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Product Information

The immunogen of 25654-1-AP is UACA Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UACA fusion protein Ag22438
• Full Name: uveal autoantigen with coiled-coil domains and ankyrin repeats
• Calculated Molecular Weight: 1416 aa, 163 kDa
• Observed Molecular Weight: 163 kDa
• GenBank Accession Number: BC113407
• Gene Symbol: UACA
• Gene ID (NCBI): 55075
• RRID: AB_2880179
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Aging (Albany NY); Transcriptome and proteome analysis of the antitumor activity of maslinic acid against pancreatic cancer cells; Authors - Hewei Zhang
human	WB	J Oncol; Maslinic Acid Inhibits the Growth of Malignant Gliomas by Inducing Apoptosis via MAPK Signaling.; Authors - Yongqiang Wang
canine	WB	Cell Rep; Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells.; Authors - Takahide Matsui
human	WB,IF	Int J Clin Exp Pathol; Knockdown of UACA inhibitsproliferation and invasion and promotes senescence of hepatocellular carcinoma cells; Authors - Jing-Yuan Sun ; KD Validated