UAP1 Polyclonal antibody

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  WB analysis using 16318-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16318-1-AP (UAP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 16318-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using UAP1 antibody (16318-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

UAP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16318-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF/ICC, ELISA

Synonyms

EC:2.7.4.-, AntigenX, Antigen X, AGX1, AGX 2

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Tested Applications

• Positive WB detected in: DU 145 cells, PC-3 cells
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16318-1-AP targets UAP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9371

  Product name: Recombinant human UAP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-352 aa of BC009377

  Sequence: MNINDLKLTLSKAGQEHLLRFWNELEEAQQVELYAELQAMNFEELNFFFQKAIEGFNQSSYQKNVDARMEPVPREVLGSATRDQDQLQAWESEGLFQISQNKVAVLLLAGGQGTRLGVAYPKGMYDVGLPSRKTLFQIQAERILKLQQVAEKYYGNKCIIPWYIMTSGRTMESTKEFFTKHKYFGLKKENVIFFQQGMLPAMSFDGKIILEEKNKVSMAPDGNGGLYRALAAQNIVEDMEQRGIWSIHVYCVDNILVKVADPRFIGFCIQKGADCGAKVVEKTNPTEPVGVVCRVDGVYQVVEYSEISLATAQKRSSDGRLLFNAGNIANHFFTVPFLRDVVNVYEPQLQHH

• Full Name: UDP-N-acteylglucosamine pyrophosphorylase 1
• Calculated Molecular Weight: 505 aa, 57 kDa
• Observed Molecular Weight: 57-59 kDa
• GenBank Accession Number: BC009377
• Gene Symbol: UAP1
• Gene ID (NCBI): 6675
• RRID: AB_2878242
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16222
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UAP1 can convert UTP and GlcNAc-1-P into UDP-GlcNAc, it can also convert UTP and GalNAc-1-P into UDP-GalNAc. There are three isoforms of UAP1, among which isoform  AGX1  has 2 to 3 times higher activity towards GalNAc-1-P, while isoform  AGX2  has 8 times more activity towards GlcNAc-1-P. It has been reported that UAP1 is specific and highly expressed in prostate cancer. UAP1 can protect cells against inhibitors of N-linked glycosylation. UAP1 may play an important role in maintaining INS responsiveness.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	J Agric Food Chem; Ginsenoside-Rb1 Improved Diabetic Cardiomyopathy through Regulating Calcium Signaling by Alleviating Protein O-GlcNAcylation.; Authors - Linhui Qin
Rat		Heliyon; Changes in transcriptomic landscape with macronutrients intake switch are independent from O-GlcNAcylation levels in heart throughout postnatal development in rats; Authors - Antoine Persello