UAP1 Monoclonal antibody, PBS Only

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  WB analysis using 67545-1-Ig (same clone as 67545-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67545-1-Ig (UAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67545-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67545-1-Ig (same clone as 67545-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67545-1-Ig (UAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67545-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67545-1-Ig (same clone as 67545-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67545-1-Ig (UAP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67545-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67545-1-Ig (same clone as 67545-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67545-1-Ig (UAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67545-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 67545-1-Ig (same clone as 67545-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using UAP1 antibody (67545-1-Ig, Clone: 2C7B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67545-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

UAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67545-1-PBS

Clone No.2C7B2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

2C7B2, AgX, AGX 2, AGX1, Antigen X

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Product Information

67545-1-PBS targets UAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9513

  Product name: Recombinant human UAP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-352 aa of BC009377

  Sequence: MNINDLKLTLSKAGQEHLLRFWNELEEAQQVELYAELQAMNFEELNFFFQKAIEGFNQSSYQKNVDARMEPVPREVLGSATRDQDQLQAWESEGLFQISQNKVAVLLLAGGQGTRLGVAYPKGMYDVGLPSRKTLFQIQAERILKLQQVAEKYYGNKCIIPWYIMTSGRTMESTKEFFTKHKYFGLKKENVIFFQQGMLPAMSFDGKIILEEKNKVSMAPDGNGGLYRALAAQNIVEDMEQRGIWSIHVYCVDNILVKVADPRFIGFCIQKGADCGAKVVEKTNPTEPVGVVCRVDGVYQVVEYSEISLATAQKRSSDGRLLFNAGNIANHFFTVPFLRDVVNVYEPQLQHH

• Full Name: UDP-N-acteylglucosamine pyrophosphorylase 1
• Calculated Molecular Weight: 505 aa, 57 kDa
• Observed Molecular Weight: 57-59 kDa
• GenBank Accession Number: BC009377
• Gene Symbol: UAP1
• Gene ID (NCBI): 6675
• RRID: AB_2882762
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16222
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

UAP1 can convert UTP and GlcNAc-1-P into UDP-GlcNAc, it can also convert UTP and GalNAc-1-P into UDP-GalNAc. There are three isoforms of UAP1, among which isoform  AGX1  has 2 to 3 times higher activity towards GalNAc-1-P, while isoform  AGX2  has 8 times more activity towards GlcNAc-1-P. It has been reported that UAP1 is specific and highly expressed in prostate cancer. UAP1 can protect cells against inhibitors of N-linked glycosylation. UAP1 may play an important role in maintaining INS responsiveness. 67545-1-Ig detects 57 and 59 kDa isoforms in SDS-PAGE.(PMID:25241896)