UBA2 Polyclonal antibody

UBA2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15347-1-AP

Synonyms

ARX, HRIHFB2115, SAE2, UBA2, UBLE1B



Tested Applications

Positive WB detected inA549 cells, human brain tissue, Jurkat cells, HeLa cells, K-562 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15347-1-AP targets UBA2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBA2 fusion protein Ag7586
Full Name ubiquitin-like modifier activating enzyme 2
Calculated molecular weight 71 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC003153
Gene symbol UBA2
Gene ID (NCBI) 10054
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UBA2 antibody 15347-1-APDownload protocol
IHC protocol for UBA2 antibody 15347-1-APDownload protocol
IF protocol for UBA2 antibody 15347-1-APDownload protocol
IP protocol for UBA2 antibody 15347-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,IHC

Cell Death Differ

Inhibition of Drp1 SUMOylation by ALR protects the liver from ischemia-reperfusion injury.

Authors - Jing Huang
  • KD Validated