Tested Applications
| Positive WB detected in | LNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH-3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68537-1-Ig targets UBA5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33685 Product name: Recombinant human UBA5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 105-404 aa of BC009737 Sequence: YDKVELANMNRLFFQPHQAGLSKVQAAEHTLRNINPDVLFEVHNYNITTVENFQHFMDRISNGGLEEGKPVDLVLSCVDNFEARMTINTACNELGQTWMESGVSENAVSGHIQLIIPGESACFACAPPLVVAANIDEKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLNFGTVSFYLGYNAMQDFFPTMSMKPNPQCDDRNCRKQQEEYKKKVAALPKQEVIQEEEEIIHEDNEWGIELVSEVSEEELKNFSGPVPDLPEGITVAYTIPKKQEDSVTELTVEDSGESLEDLMAKMKNM Predict reactive species |
| Full Name | ubiquitin-like modifier activating enzyme 5 |
| Calculated Molecular Weight | 404 aa, 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC009737 |
| Gene Symbol | UBA5 |
| Gene ID (NCBI) | 79876 |
| RRID | AB_3085245 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9GZZ9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UBA5 antibody 68537-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UBA5 Monoclonal antibody (3E5C8), catalog number 68537-1-Ig, has 1 total citation. The citation appears in J Virol (Journal of Virology), published in 2026. The associated research field is virology, specifically examining how UFMylation of SHBs (small hydrophobic basic proteins) increases protein stability by antagonizing ubiquitination and promotes virion production.
| Species | Application | Title |
|---|---|---|
J Virol UFMylation of SHBs increases protein stability by antagonizing ubiquitination and promotes virion production. |
