UBE1L Polyclonal antibody

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• UBE1L Polyclonal antibody (15818-1-AP)

UBE1L Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15818-1-AP Copied!

Publications (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

UBA7, D8, EC:6.2.1.-, UBA 7, UBA1B

• 

  WB analysis using 15818-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15818-1-AP (UBE1L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 15818-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15818-1-AP (UBE1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15818-1-AP targets UBE1L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8692

  Product name: Recombinant human UBA7 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 661-1012 aa of BC006378

  Sequence: VRPQNWQDCVAWALGHWKLCFHYGIKQLLRHFPPNKVLEDGTPFWSGPKQCPQPLEFDTNQDTHLLYVLAAANLYAQMHGLPGSQDWTALRELLKLLPQPDPQQMAPIFASNLELASASAEFGPEQQKELNKALEVWSVGPPLKPLMFEKDDDSNFHVDFVVAAASLRCQNYGIPPVNRAQSKRIVGQIIPAIATTTAAVAGLLGLELYKVVSGPRPRSAFRHSYLHLAENYLIRYMPFAPAIQTFHHLKWTSWDRLKVPAGQPERTLESLLAHLQEQHGLRVRILLHGSALLYAAGWSPEKQAQHLPLRVTELVQQLTGQAPAPGQRVLVLELSCEGDDEDTAFPPLHYEL

• Full Name: ubiquitin-like modifier activating enzyme 7
• Calculated Molecular Weight: 1012 aa, 112 kDa
• Observed Molecular Weight: 112 kDa
• GenBank Accession Number: BC006378
• Gene Symbol: UBE1L
• Gene ID (NCBI): 7318
• RRID: AB_2211764
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P41226
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The UBE1L (UBA7) Polyclonal Antibody (Cat# 15818-1-AP) has 5 total citations. These appear in Nature Communications, Cell Death & Disease, International Journal of Molecular Medicine, Free Radical Biology and Medicine, and Cell Reports. Associated research fields include ISGylation and ubiquitin ligase activation, autophagy regulation, cancer cell apoptosis, ferroptosis in hepatocellular carcinoma, and RIPK3-mediated cell death in viral pathogenesis.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Nat Commun; ATP functions as a pathogen-associated molecular pattern to activate the E3 ubiquitin ligase RNF213; Authors - Juraj Ahel
human	WB	Cell Death Dis; Induced TRIM21 ISGylation by IFN-β enhances p62 ubiquitination to prevent its autophagosome targeting.; Authors - Jie Jin
human	WB	Int J Mol Med; ISG15 inhibits cancer cell growth and promotes apoptosis.; Authors - Mei-Juan Zhou
		Free Radic Biol Med; TBX15 increases ferroptosis to overcome sorafenib resistance via HERC5-mediated cGAS ISGylation in hepatocellular carcinoma.; Authors - Hui Yuan
human	WB	Cell Rep; ISG15 negatively regulates RIPK3-mediated cell death and viral pathogenesis.; Authors - Yi-Chieh Perng