UBAC1 Polyclonal antibody

UBAC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19844-1-AP

Synonyms

GBDR1, KPC2, RP11 432J22.3, UBA domain containing 1, UBAC1, UBADC1

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Validation Data Gallery

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  WB analysis using 19844-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19844-1-AP (UBAC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 19844-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19844-1-AP (UBAC1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 19844-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19844-1-AP (UBAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 19844-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19844-1-AP (UBAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 19844-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19844-1-AP (UBAC1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat liver tissue, mouse heart tissue, rat brain tissue, rat heart tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19844-1-AP is UBAC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBAC1 fusion protein Ag13887
• Full Name: UBA domain containing 1
• Calculated Molecular Weight: 405 aa, 45 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC004967
• Gene Symbol: UBAC1
• Gene ID (NCBI): 10422
• RRID: AB_10638923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBAC1(Ubiquitin-associated domain-containing protein 1) is also named as GBDR1, KPC2, UBADC1. It has several putative phosphorylation sites, including 8 CK2 (see 115440) sites, as well as 1 N-glycosylation site and 2 N-myristoylation sites(PMID:10857748). It is required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.Northern blot analysis shows UBAC1 expression with the highest levels in heart and skeletal muscle and lowest level in lung. This antibody is specific to UBAC1.