UBAC1 Monoclonal antibody

UBAC1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2A3G7

Cat no : 67385-1-Ig

Synonyms

KPC2, UBA domain containing 1, Kip1 ubiquitination-promoting complex protein 2, Glialblastoma cell differentiation-related protein 1, GBDR1

---

Validation Data Gallery

• 

  WB analysis using 67385-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67385-1-Ig (UBAC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  IF Staining of HeLa using 67385-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using UBAC1 antibody (67385-1-Ig, Clone: 2A3G7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

---

Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, pig brain tissue, mouse brain tissue, rabbit brain tissue, rat brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• CoIP: See 1 publications below

Product Information

67385-1-Ig targets UBAC1 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: UBAC1 fusion protein Ag13958
• Full Name: UBA domain containing 1
• Calculated Molecular Weight: 405 aa, 45 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC004967
• Gene Symbol: UBAC1
• Gene ID (NCBI): 10422
• RRID: AB_2882630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBAC1(Ubiquitin-associated domain-containing protein 1) is also named as GBDR1, KPC2, UBADC1. It has several putative phosphorylation sites, including 8 CK2 (see 115440) sites, as well as 1 N-glycosylation site and 2 N-myristoylation sites(PMID:10857748). It is required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.Northern blot analysis shows UBAC1 expression with the highest levels in heart and skeletal muscle and lowest level in lung.

Publications

Species	Application	Title
human	WB,CoIP	Cell Host Microbe; Human cytomegalovirus degrades DMXL1 to inhibit autophagy, lysosomal acidification, and viral assembly; Authors - Hanqi Li