UBAC1 Monoclonal antibody
UBAC1 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig, rabbit
Applications
WB, IF/ICC, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
2A3G7
Cat no : 67385-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, pig brain tissue, mouse brain tissue, rabbit brain tissue, rat brain tissue |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
CoIP | See 1 publications below |
Product Information
67385-1-Ig targets UBAC1 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
Tested Reactivity | human, mouse, rat, pig, rabbit |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | UBAC1 fusion protein Ag13958 |
Full Name | UBA domain containing 1 |
Calculated Molecular Weight | 405 aa, 45 kDa |
Observed Molecular Weight | 46 kDa |
GenBank Accession Number | BC004967 |
Gene Symbol | UBAC1 |
Gene ID (NCBI) | 10422 |
RRID | AB_2882630 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
UBAC1(Ubiquitin-associated domain-containing protein 1) is also named as GBDR1, KPC2, UBADC1. It has several putative phosphorylation sites, including 8 CK2 (see 115440) sites, as well as 1 N-glycosylation site and 2 N-myristoylation sites(PMID:10857748). It is required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.Northern blot analysis shows UBAC1 expression with the highest levels in heart and skeletal muscle and lowest level in lung.
Protocols
Product Specific Protocols | |
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WB protocol for UBAC1 antibody 67385-1-Ig | Download protocol |
IF protocol for UBAC1 antibody 67385-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Host Microbe Human cytomegalovirus degrades DMXL1 to inhibit autophagy, lysosomal acidification, and viral assembly |