UBAP1 Polyclonal antibody

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UBAP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 12385-1-AP Copied!

Publications (18)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

NAG20, Nasopharyngeal carcinoma-associated gene 20 protein, UAP, UBAP, UBAP 1

• 

  WB analysis using 12385-1-AP

  A431 cell lysates were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 12385-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 12385-1-AP

  IP result of anti-UBAP1 (IP:12385-1-AP, 4ug; Detection:12385-1-AP 1:2000) with mouse brain tissue lysate 1680 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 12385-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using UBAP1 antibody (12385-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12385-1-AP targets UBAP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3055

  Product name: Recombinant human UBAP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 203-502 aa of BC020950

  Sequence: GGSGSVLQDEEVLASLERATLDFKPLHKPNGFITLPQLGNCEKMSLSSKVSLPPIPAVSNIKSLSFPKLDSDDSNQKTAKLASTFHSTSCLRNGTFQNSLKPSTQSSASELNGHHTLGLSALNLDSGTEMPALTSSQMPSLSVLSVCTEESSPPNTGPTVTPPNFSVSQVPNMPSCPQAYSELQMLSPSERQCVETVVNMGYSYECVLRAMKKKGENIEQILDYLFAHGQLCEKGFDPLLVEEALEMHQCSEEKMMEFLQLMSKFKEMGFELKDIKEVLLLHNNDQDNALEDLMARAGAS

• Full Name: ubiquitin associated protein 1
• Calculated Molecular Weight: 502 aa, 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC020950
• Gene Symbol: UBAP1
• Gene ID (NCBI): 51271
• RRID: AB_2211886
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NZ09
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBAP1, also named as NAG20, is a 55 kDa protein which contains 2 putative tandem UBA domains at the C terminus, a coiled-coil domain, 2 possible N-glycosylation sites, 5 PKC phosphorylation sites, 7 casein kinase II phosphorylation sites, and 3 N-myristoylation sites. UBAP1 might be a potential effective diagnosis candidate for NPC and decreased expression of UBAP1 protein is a possible point of dysfunction along the pathogenesis pathway for NPC that may contribute to malignant transformation.

Publications

What published studies show

The anti-UBAP1 antibody (Cat# 12385-1-AP) has 18 citations published in journals including Cell Reports, EMBO Journal, Current Biology, Journal of Cell Biology, PLoS Pathogens, Structure, iScience, Journal of Biological Chemistry, Journal of Cell Science, Science Signaling, RSC Chemical Biology, and Carcinogenesis. Research fields span ESCRT-mediated endosomal sorting, autophagosome closure, neurogenesis, cytokine receptor trafficking, HIV virology, and cancer biology.

Automatically generated from the 18 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	EMBO J; A Pseudomonas aeruginosa TIR effector mediates immune evasion by targeting UBAP1 and TLR adaptors.; Authors - Paul Rc Imbert
human	WB	Cell Rep; The Dynamics of TGF-β Signaling Are Dictated by Receptor Trafficking via the ESCRT Machinery.; Authors - Daniel S J Miller
mouse	WB,IF	Cell Rep; ESCRT-I protein UBAP1 controls ventricular expansion and cortical neurogenesis via modulating adherens junctions of radial glial cells; Authors - Danping Lu
human	WB,IP	Cell Rep; Interplay of Endosomal pH and Ligand Occupancy in Integrin α5β1 Ubiquitination, Endocytic Sorting, and Cell Migration.; Authors - Dmitri Kharitidi
mouse,human	WB	Cell Rep; Unveiling the physiological impact of ESCRT-dependent autophagosome closure by targeting the VPS37A ubiquitin E2 variant-like domain; Authors - Kouta Hamamoto
human	WB	Curr Biol; UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.; Authors - Stefani Flavia F