UBAP1 Monoclonal antibody, PBS Only

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  WB analysis using 66993-1-Ig (same clone as 66993-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66993-1-Ig (UBAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66993-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66993-1-Ig (same clone as 66993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66993-1-Ig (UBAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66993-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66993-1-Ig (same clone as 66993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66993-1-Ig (UBAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66993-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66993-1-Ig (same clone as 66993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66993-1-Ig (UBAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66993-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66993-1-Ig (same clone as 66993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66993-1-Ig (UBAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66993-1-PBS in a different storage buffer formulation.

UBAP1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66993-1-PBS

Clone No.2A6A2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Synonyms

NAG20, UAP, UBAP, UBAP 1, UBAP1, ubiquitin associated protein 1

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Product Information

66993-1-PBS targets UBAP1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17675

  Product name: Recombinant human UBAP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 203-502 aa of BC020950

  Sequence: GGSGSVLQDEEVLASLERATLDFKPLHKPNGFITLPQLGNCEKMSLSSKVSLPPIPAVSNIKSLSFPKLDSDDSNQKTAKLASTFHSTSCLRNGTFQNSLKPSTQSSASELNGHHTLGLSALNLDSGTEMPALTSSQMPSLSVLSVCTEESSPPNTGPTVTPPNFSVSQVPNMPSCPQAYSELQMLSPSERQCVETVVNMGYSYECVLRAMKKKGENIEQILDYLFAHGQLCEKGFDPLLVEEALEMHQCSEEKMMEFLQLMSKFKEMGFELKDIKEVLLLHNNDQDNALEDLMARAGAS

• Full Name: ubiquitin associated protein 1
• Calculated Molecular Weight: 502 aa, 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC020950
• Gene Symbol: UBAP1
• Gene ID (NCBI): 51271
• RRID: AB_2882310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9NZ09
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

UBAP1, also named as NAG20, is a 55 kDa protein which contains 2 putative tandem UBA domains at the C terminus, a coiled-coil domain, 2 possible N-glycosylation sites, 5 PKC phosphorylation sites, 7 casein kinase II phosphorylation sites, and 3 N-myristoylation sites. UBAP1 might be a potential effective diagnosis candidate for NPC and decreased expression of UBAP1 protein is a possible point of dysfunction along the pathogenesis pathway for NPC that may contribute to malignant transformation.