UBAP2L Monoclonal antibody

• 

  WB analysis of K-562 using 67588-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67588-1-Ig (UBAP2L antibody) at dilution of 1:1700 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67588-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67588-1-Ig (UBAP2L antibody) at dilution of 1:1700 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 67588-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67588-1-Ig (UBAP2L antibody) at dilution of 1:1700 incubated at room temperature for 1.5 hours.

UBAP2L Monoclonal Antibody for WB, ELISA

Cat No. 67588-1-Ig

Clone No.1E3D1

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Synonyms

1E3D1, KIAA0144, NICE 4, NICE4, Protein NICE 4

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67588-1-Ig targets UBAP2L in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: UBAP2L fusion protein Ag30125
• Full Name: ubiquitin associated protein 2-like
• Calculated Molecular Weight: 115 kDa
• Observed Molecular Weight: 140-160 kDa
• GenBank Accession Number: BC003170
• Gene Symbol: UBAP2L
• Gene ID (NCBI): 9898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14157
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin-associated protein 2-like (UBAP2L) is a highly conserved protein with an N-terminal ubiquitin-associated (UBA) domain involved in the ubiquitin-proteasome system and aggregate formation induced by proteasome inhibitors. It was originally identified as a human sperm protein that interacts with zona pellucida 3 in human eggs. It can interact with BIM1 to form a complex that modulates hematopoietic stem cell activity. Recent studies have confirmed that UBAP2L function as an oncogene and is associated with various types of cancer, including prostate cancer, glioma, hepatocellular carcinoma (HCC), and colorectal carcinoma. (PMID: 31114027, PMID: 29196913)