UBASH3B/STS 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

UBASH3B/STS 1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 19563-1-AP

Synonyms

Cbl interacting protein p70, KIAA1959, p70, STS 1, STS1, UBASH3B, UBASH3B/STS 1

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Validation Data Gallery

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  WB analysis of HEK-293 using 19563-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19563-1-AP (UBASH3B/STS 1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19563-1-AP

  WB result of UBASH3B/STS 1 antibody (19563-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBASH3B/STS 1 transfected HEK-293 cells.

• 

  WB analysis of mouse lung using 19563-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19563-1-AP (UBASH3B/STS 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 19563-1-AP

  IP result of anti-UBASH3B/STS 1 (IP:19563-1-AP, 3ug; Detection:19563-1-AP 1:300) with mouse kidney tissue lysate 6000ug.

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  IHC staining of human hepatocirrhosis using 19563-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19563-1-AP (UBASH3B/STS 1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 19563-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19563-1-AP (UBASH3B/STS 1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 19563-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19563-1-AP (UBASH3B/STS 1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 19563-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19563-1-AP (UBASH3B/STS 1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse lung tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human hepatocirrhosis tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

19563-1-AP targets UBASH3B/STS 1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ubiquitin associated and SH3 domain containing, B
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: NM_032873
• Gene Symbol: UBASH3B
• Gene ID (NCBI): 84959
• RRID: AB_10643379
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBASH3B, also named STS1 or TULA-2, is a protein that contains a ubiquitin-associated domain at the N-terminus, a SH3 domain, and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. TULA-2 is supposed to form homodimer and interact with ubiquitin and mono-ubiquitinated proteins. TULA-2 functions to promote accumulation of activated target receptors, such as T-cell receptors and EGFR on the cell surface.

Publications

Species	Application	Title
human	WB	Cell; Oncogenic Mutations Rewire Signaling Pathways by Switching Protein Recruitment to Phosphotyrosine Sites.; Authors - Alicia Lundby
human,mouse	WB	Biochimie; Ubash3b promotes TPA-mediated suppression of leukemogenesis through accelerated downregulation of PKCδ protein.; Authors - Yao Yao ; KD Validated
mouse	WB	Front Immunol; Targeting the Cbl-b-Notch1 axis as a novel immunotherapeutic strategy to boost CD8+ T-cell responses; Authors - Giulia Monticone ; KO Validated
human	WB	Int J Mol Sci; STS1 and STS2 Phosphatase Inhibitor Baicalein Enhances the Expansion of Hematopoietic and Progenitor Stem Cells and Alleviates 5-Fluorouracil-Induced Myelosuppression; Authors - Na Li
human	WB	Transl Cancer Res; Prazosin inhibits the growth and mobility of osteosarcoma cells; Authors - Ming An
human,mouse	WB,IF	Cell Rep; PSGL-1 attenuates early TCR signaling to suppress CD8+ T cell progenitor differentiation and elicit terminal CD8+ T cell exhaustion; Authors - Jennifer L Hope