Tested Applications
| Positive IHC detected in | mouse liver tissue, human lung cancer tissue, human urothelial carcinoma tissue, mouse stomach tissue, rat liver tissue, rat pancreas tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10457-1-AP targets UBC in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0711 Product name: Recombinant human UBC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 14-265 aa of BC008955 Sequence: MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE Predict reactive species |
| Full Name | ubiquitin C |
| Calculated Molecular Weight | 60 kDa |
| GenBank Accession Number | BC008955 |
| Gene Symbol | UBC |
| Gene ID (NCBI) | 7316 |
| RRID | AB_2241301 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P0CG48 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ubiquitin (Ub) is a small, highly conserved eukaryotic protein that has an essential role in diverse cellular signaling pathways, including targeting proteins for proteasomal degradation. Cellular Ub is comprised of two distinct pools consisting of free Ub and Ub-substrate conjugates. UbC is a polyubiquitine gene that encodes Ub polyprotein. UbC, as stress-inducible polyubiquitin precursor proteins of around 9-11 monomers, is cleaved into monomeric ubiquitin by cellular deubiquitylating enzymes.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UBC antibody 10457-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UBC Polyclonal antibody (catalog no. 10457-1-AP) has 7 citations. These appear in Frontiers in Pharmacology, Chemical & Biological Interactions, PLoS Pathogens, Neuroscience, PLoS ONE, and bioRxiv. The associated research fields span pulmonary fibrosis, apoptosis, mitochondrial dynamics and oxidative stress, neuroinflammation, cervical cancer stem cells, allergic rhinitis, and hepatic lipid metabolism.
| Species | Application | Title |
|---|---|---|
Front Pharmacol Diacetyl-induced bronchiolitis obliterans involves ubiquitin C upregulation and fibrosis-related gene activation in rats. | ||
Chem Biol Interact Proteomic analysis of apoptosis induction by lariciresinol in human HepG2 cells. | ||
PLoS Pathog The UBC/SIRT5/DRP1 axis regulates mitochondrial dynamics to alleviate Staphylococcus aureus-induced oxidative stress and senescence in bovine mammary epithelial cells. | ||
Neuroscience Maresin 1 Activates LGR6 to Alleviate Neuroinflammation via the CREB/JMJD3/IRF4 Pathway in a Rat Model of Subarachnoid Hemorrhage | ||
PLoS One Ubiquitin B in cervical cancer: critical for the maintenance of cancer stem-like cell characters.
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PLoS One UBC/UBA52 silencing restores PINK1-Parkin-mediated mitochondrial autophagy in allergic rhinitis.
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Reviews
What customers say
The single review for this product is highly positive, with a 5-star rating. The reviewer used the antibody for immunohistochemistry and reported that it worked well, describing the performance as reliable and satisfactory for their application. Overall, the feedback indicates the reagent performed as expected in IHC experiments.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
wei (Verified Customer) (07-24-2025) | USED WORK WELL
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