UBE2G2 Polyclonal antibody

UBE2G2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10722-1-AP

Synonyms

UBC7, UBE2G2, Ubiquitin carrier protein G2, Ubiquitin protein ligase G2

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Validation Data Gallery

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  WB analysis of Jurkat using 10722-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10722-1-AP (UBE2G2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10722-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10722-1-AP (UBE2G2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10722-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10722-1-AP (UBE2G2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10722-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10722-1-AP (UBE2G2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 10722-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10722-1-AP (UBE2G2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10722-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10722-1-AP (UBE2G2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, K-562 cells, mouse testis tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10722-1-AP targets UBE2G2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBE2G2 fusion protein Ag1082
• Full Name: ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast)
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 16-19 kDa
• GenBank Accession Number: BC008351
• Gene Symbol: UBE2G2
• Gene ID (NCBI): 7327
• RRID: AB_2256726
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.