UBC9-Specific Polyclonal antibody

UBC9-Specific Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 14837-1-AP

Synonyms

C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I



Tested Applications

Positive WB detected inHEK-293 cells, human testis tissue, Jurkat cells, U-937 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

14837-1-AP targets UBC9-Specific in WB, IHC, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberNM_003345
Gene symbol UBE2I
Gene ID (NCBI) 7329
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).

Protocols

Product Specific Protocols
WB protocol for UBC9-Specific antibody 14837-1-APDownload protocol
IHC protocol for UBC9-Specific antibody 14837-1-APDownload protocol
FC protocol for UBC9-Specific antibody 14837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Praveen (Verified Customer) (03-13-2019)

Excellent

  • Applications: Western Blot,
  • Cell Tissue Type: AC16
FH

Shubham (Verified Customer) (03-12-2019)

Very good antibody with a prominent band.

  • Applications: Western Blot,
  • Cell Tissue Type: AC16
UBC9-Specific Antibody Western Blot, validation ( dilution) in AC16 (Cat no:14837-1-AP)