Validation Data Gallery
|Positive WB detected in||HEK-293 cells, human testis tissue, Jurkat cells, U-937 cells|
|Positive IHC detected in||human breast cancer tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
14837-1-AP targets UBC9-Specific in WB, IHC, IF, FC applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)|
|Calculated molecular weight||18 kDa|
|Observed molecular weight||18 kDa|
|GenBank accession number||NM_003345|
|Gene ID (NCBI)||7329|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Praveen (Verified Customer) (03-13-2019)
Shubham (Verified Customer) (03-12-2019)
Very good antibody with a prominent band.