Tested Applications
| Positive WB detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17713-1-AP targets UBE2J2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12039 Product name: Recombinant human UBE2J2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-226 aa of BC094777 Sequence: MSSTSSKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKRQLAVQSLAFNLKDKVFCELFPEVVEEIKQKQKAQDELSSRPQTLPLPDVVPDGETHLVQNGIQLLNGHAPGAVPNLAGLQQANRHH Predict reactive species |
| Full Name | ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast) |
| Calculated Molecular Weight | 259 aa, 29 kDa |
| Observed Molecular Weight | 29 kDa |
| GenBank Accession Number | BC094777 |
| Gene Symbol | UBE2J2 |
| Gene ID (NCBI) | 118424 |
| RRID | AB_2210754 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N2K1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UBE2J2 antibody 17713-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UBE2J2 Polyclonal antibody (Cat# 17713-1-AP) has 4 citations. These appear in Molecular Cell, Developmental Cell, EMBO Reports, and American Journal of Cancer Research. The associated research fields include mTORC1 signaling, lipid droplet regulation, protein quality control, and UBE2J2-mediated ubiquitination in cervical cancer. All cited applications were Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Dev Cell Parallel CRISPR-Cas9 screens identify mechanisms of PLIN2 and lipid droplet regulation | ||
EMBO Rep MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins. | ||
Am J Cancer Res UBE2J2-mediated ubiquitination modification of MCM6 regulates proliferation and migration of cervical cancer cells. |
Reviews
What customers say
The single review rates the antibody 3 out of 5. The user tested it on HeLa cells by Western Blot at a 1:2000 dilution and described it as a "pretty good" antibody. While it showed some background bands, the specific band was clearly visible and was confirmed to deplete upon knockout, supporting its target specificity. Overall, the feedback is moderately positive, noting acceptable performance with minor background issues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Anna (Verified Customer) (07-17-2019) | Pretty good antibody. Quite a few background bands, but can see specific band depletes with knockout.
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