|Positive WB detected in||mouse thymus tissue, human heart tissue, Jurkat cells, mouse small intestine tissue|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10243-1-AP targets UBC13 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||UBC13 fusion protein Ag0292|
|Full Name||ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)|
|Calculated molecular weight||17 kDa|
|Observed molecular weight||17 kDa|
|GenBank accession number||BC000396|
|Gene ID (NCBI)||7334|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2N is a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jessica (Verified Customer) (11-14-2019)
Antibody works well for detecting the endogenous Ubc13 protein in 293T cells. Strong signal and few non specific bands.