UBE2S Monoclonal antibody
UBE2S Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
8C9E6
Cat no : 66897-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, Jurkat cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66897-1-Ig targets UBE2S in WB, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | UBE2S fusion protein Ag5378 |
Full Name | ubiquitin-conjugating enzyme E2S |
Calculated Molecular Weight | 222 aa, 24 kDa |
Observed Molecular Weight | 28 kDa |
GenBank Accession Number | BC066948 |
Gene Symbol | UBE2S |
Gene ID (NCBI) | 27338 |
RRID | AB_2882226 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).
Protocols
Product Specific Protocols | |
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WB protocol for UBE2S antibody 66897-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |