UBE3C Polyclonal antibody

UBE3C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12333-1-AP

Synonyms

KIAA10, KIAA0010, Homologous to E6AP carboxyl terminus homologous protein 2, HECT-type ubiquitin transferase E3C, HectH2

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Validation Data Gallery

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  WB analysis using 12333-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12333-1-AP (UBE3C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12333-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12333-1-AP (UBE3C Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 12333-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12333-1-AP (UBE3C antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12333-1-AP targets UBE3C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBE3C fusion protein Ag2966
• Full Name: ubiquitin protein ligase E3C
• Calculated Molecular Weight: 124 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC026241
• Gene Symbol: UBE3C
• Gene ID (NCBI): 9690
• RRID: AB_2877846
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	Int Dent J; LncRNA AFAP1-AS1 Promotes Oral Squamous Cell Carcinoma Development by Ubiquitin-Mediated Proteolysis; Authors - Bao-Jun Li