UBE3C Polyclonal antibody

• Home
• Products
• Primary Antibodies
• UBE3C Polyclonal antibody (12333-1-AP)

UBE3C Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12333-1-AP Copied!

Publications (4)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EC:2.3.2.26, HectH2, HECT-type ubiquitin transferase E3C, Homologous to E6AP carboxyl terminus homologous protein 2, KIAA0010

• 

  WB analysis using 12333-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12333-1-AP (UBE3C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 12333-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12333-1-AP (UBE3C Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 12333-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12333-1-AP (UBE3C antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for UBE3C in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12333-1-AP targets UBE3C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2966

  Product name: Recombinant human UBE3C protein

  Source: e coli.-derived, T-GST

  Tag: GST

  Domain: 1-350 aa of BC026241

  Sequence: MFSFEGDFKTRPKVSLGGASRKYSIQRSAFDRCATLSQSGGAFPIANGPNLTLLVRQLLFFYKQNEDSKRLIWLYQNLIKHSSLFVKQLDGSERLTCLFQIKRLMSLCCRLLQNCNDDSLNVALPMRMLEVFSSENTYLPVLQDASYVVSVIEQILHYMIHNGYYRSLYLLINSKLPSSIEYSDLSRVPIAKILLENVLKPLHFTYNSCPEGARQQVFTAFTEEFLAAPFTDQIFHFIIPALADAQTVFPYEPFLNALLLIESRCSRKSGGAPWLFYFVLTVGENYLGALSEEGLLVYLRVLQTFLSQLPVSPASASCHDSASDSEEESEEADKPSSPEDGRLSVSYITE

• Full Name: ubiquitin protein ligase E3C
• Calculated Molecular Weight: 124 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC026241
• Gene Symbol: UBE3C
• Gene ID (NCBI): 9690
• RRID: AB_2877846
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15386
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

12333-1-AP (UBE3C Antibody) has 4 total citations published in Int Dent J, Sci Rep, Acta Biochim Biophys Sin (Shanghai), and Mol Biol Rep. The associated research fields include cancer biology (oral squamous cell carcinoma, pancreatic ductal adenocarcinoma), oncogene regulation (MYC), RNA modification (m6A/YTHDF2), and ovarian aging. All citations utilized WB, with one also employing IHC, across human and mouse species.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB	Int Dent J; LncRNA AFAP1-AS1 Promotes Oral Squamous Cell Carcinoma Development by Ubiquitin-Mediated Proteolysis; Authors - Bao-Jun Li
human	WB	Sci Rep; Endogenous protein tagging coupled with a CRISPR screening approach identifies UBE3C as a potential MYC oncogene regulator.; Authors - Marcel Seibert
mouse	WB	Acta Biochim Biophys Sin (Shanghai); Melatonin mitigates ovarian aging through regulation of the YTHDF2/m6A/UBE3C axis; Authors - Wenjuan Xia
human	WB,IHC	Mol Biol Rep; UBE3C promotes pancreatic ductal adenocarcinoma progression by catalysing p53 ubiquitination; Authors - Yang Shi ; KD Validated