UBN1-Specific Polyclonal antibody

UBN1-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20363-1-AP

Synonyms

HIRA binding protein, Protein VT4, ubinuclein 1, UBN1, UBN1-Specific, VT, VT4

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Validation Data Gallery

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  WB analysis of mouse liver using 20363-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20363-1-AP (UBN1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 20363-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20363-1-AP (UBN1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 20363-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20363-1-AP (UBN1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 20363-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20363-1-AP (UBN1-Specific antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, MCF-7 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

20363-1-AP targets UBN1-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ubinuclein 1
• Calculated Molecular Weight: 121 kDa
• Observed Molecular Weight: 120-140 kDa
• GenBank Accession Number: NM_016936
• Gene Symbol: UBN1
• Gene ID (NCBI): 29855
• RRID: AB_10694818
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBN1, also named as Protein VT4, belongs to the ubinuclein family. UBN1 acts as a novel regulator of senescence. It is involved in the formation of senescence-associated heterochromatin foci (SAHF), which represses expression of proliferation-promoting genes. UBN1 binds to proliferation-promoting genes. It may be required for replication-independent chromatin assembly. The antibody is specific to UBN1.

Publications

Species	Application	Title
human	WB	Cell Stem Cell; PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs.; Authors - Zhexin Zhu