Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13568-1-AP targets UBQLN3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4462 Product name: Recombinant human Ubiquilin 3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 305-655 aa of BC036743 Sequence: PWTSTHGGSGSRQGRQDGDQDAPDIRNRFPNFLGIIRLYDYLQQLHENPQSLGTYLQGTASALSQSQEPPPSVNRVPPSSPSSQEPGSGQPLPEESVAIKGRSSCPAFLRYPTENSTGQGGDQDGAGKSSTGHSTNLPDLVSGLGDSANRVPFAPLSFSPTAAIPGIPEPPWLPSPAYPRSLRPDGMNPAPQLQDEIQPQLPLLMHLQAAMANPRALQALRQIEQGLQVLATEAPRLLLWFMPCLAGTGSVAGGIESREDPLMSEDPLPNPPPEVFPALDSAELGFLSPPFLHMLQDLVSTNPQQLQPEAHFQVQLEQLRSMGFLNREANLQALIATGGDVDAAVEKLRQS Predict reactive species |
| Full Name | ubiquilin 3 |
| Calculated Molecular Weight | 655 aa, 71 kDa |
| Observed Molecular Weight | 71 kDa |
| GenBank Accession Number | BC036743 |
| Gene Symbol | UBQLN3 |
| Gene ID (NCBI) | 50613 |
| RRID | AB_2241124 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H347 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
UBQLN3(Ubiquilin-3) may interact with the testis-specific cyclin A1 and regulate cell cycle progression during spermatogenesis, as does frog Xdrp1, with which UBQLN3 shares sequence similarity(PMID:10831842).UBQLN3 expression is not observed in mouse eye, thyroid, pancreas,smooth muscle, ovary, submaxillary gland, uterus, and epididymis by northern blot(PMID:10831842 ).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for UBQLN3 antibody 13568-1-AP | Download protocol |
| WB protocol for UBQLN3 antibody 13568-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UBQLN3 Polyclonal antibody (Cat# 13568-1-AP) has 2 citations. These appear in Fertility and Sterility and Molecular Reproduction and Development. The research fields covered include reproductive medicine and spermatogenesis—specifically, identification of potential biomarkers for nonobstructive azoospermia via microarray gene expression analysis, and investigation of UBQLN3's role as a testis-specific gene in embryonic development and spermatogenesis in mice.
| Species | Application | Title |
|---|---|---|
Fertil Steril Potential biomarkers of nonobstructive azoospermia identified in microarray gene expression analysis. | ||
Mol Reprod Dev Ubqln3, a testis-specific gene, is dispensable for embryonic development and spermatogenesis in mice. |
