UBQLN3 Polyclonal antibody

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  WB analysis of mouse testis using 23372-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 23372-1-AP (UBQLN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 23372-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23372-1-AP (UBQLN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 23372-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 23372-1-AP (UBQLN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 23372-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23372-1-AP (UBQLN3 antibody at dilution of 1:50.

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  IHC staining of human testis using 23372-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23372-1-AP (UBQLN3 antibody at dilution of 1:50.

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  IF Staining of HepG2 using 23372-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UBQLN3 antibody (23372-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

UBQLN3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 23372-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

UBQLN 3, Ubiquilin-3, ubiquilin3, ubiquilin 3, TUP1

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Tested Applications

• Positive WB detected in: mouse testis tissue, HepG2 cells, human testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23372-1-AP targets UBQLN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19982

  Product name: Recombinant human Ubiquilin 3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 306-620 aa of BC036743

  Sequence: WTSTHGGSGSRQGRQDGDQDAPDIRNRFPNFLGIIRLYDYLQQLHENPQSLGTYLQGTASALSQSQEPPPSVNRVPPSSPSSQEPGSGQPLPEESVAIKGRSSCPAFLRYPTENSTGQGGDQDGAGKSSTGHSTNLPDLVSGLGDSANRVPFAPLSFSPTAAIPGIPEPPWLPSPAYPRSLRPDGMNPAPQLQDEIQPQLPLLMHLQAAMANPRALQALRQIEQGLQVLATEAPRLLLWFMPCLAGTGSVAGGIESREDPLMSEDPLPNPPPEVFPALDSAELGFLSPPFLHMLQDLVSTNPQQLQPEAHFQVQL

• Full Name: ubiquilin 3
• Calculated Molecular Weight: 655 aa, 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC036743
• Gene Symbol: UBQLN3
• Gene ID (NCBI): 50613
• RRID: AB_2879268
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H347
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBQLN3(Ubiquilin-3) may interact with the testis-specific cyclin A1 and regulate cell cycle progression during spermatogenesis, as does frog Xdrp1, with which UBQLN3 shares sequence similarity(PMID:10831842).UBQLN3 expression is not observed in mouse eye, thyroid, pancreas,smooth muscle, ovary, submaxillary gland, uterus, and epididymis by northern blot(PMID:10831842 ).