UBQLNL Polyclonal antibody

UBQLNL Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16400-1-AP

Synonyms

Ubiquilin-like protein, Ubiquilin like protein, ubiquilin like

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Validation Data Gallery

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  WB analysis of human brain using 16400-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16400-1-AP (UBQLNL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16400-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16400-1-AP (UBQLNL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 16400-1-AP

  IP result of anti-UBQLNL (IP:16400-1-AP, 4ug; Detection:16400-1-AP 1:500) with Jurkat cells lysate 3200ug.

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  IHC staining of human colon using 16400-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16400-1-AP (UBQLNL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 16400-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16400-1-AP (UBQLNL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 16400-1-AP

  Immunofluorescent analysis of A431 cells using 16400-1-AP (UBQLNL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of A431 using 16400-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using UBQLNL antibody (16400-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: human brain tissue, Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

16400-1-AP targets UBQLNL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBQLNL fusion protein Ag9496
• Full Name: ubiquilin-like
• Calculated Molecular Weight: 475 aa, 53 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC012183
• Gene Symbol: UBQLNL
• Gene ID (NCBI): 143630
• RRID: AB_2878253
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBQLNL also named as Ubiquilin like protein, encodes a 475 amino acid protein. The specific function of it is still non-known. UBQLNL may have an association with type 2 diabetes, but the mechanism is supposed be studied in the further research. The subcellular localization of UBQLNL is nucleus. The expression of it is detected in some tissues, such as brain ,testis, heart and liver.

Publications

Species	Application	Title
mouse	WB,IF	Mol Med Rep; Decreased expression of ubiquilin‑1 following neonatal hypoxia‑ischemic brain injury in mice.; Authors - Li Luo