UBR5 Polyclonal antibody

UBR5 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 22782-1-AP

Publications(7)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

EDD1, EDD, EC:2.3.2.26, E3 ubiquitin-protein ligase, HECT domain-containing 1, E3 ubiquitin-protein ligase UBR5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllIHCIF/ICC

IHC staining of mouse testis using 22782-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22782-1-AP (UBR5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 22782-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22782-1-AP (UBR5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 22782-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22782-1-AP (UBR5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 22782-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22782-1-AP (UBR5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 22782-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22782-1-AP (UBR5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 22782-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22782-1-AP (UBR5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of MCF-7 using 22782-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 22782-1-AP (UBR5 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (7)
Protocols

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MSDS SDS

Tested Applications

Positive IHC detected in	mouse testis tissue, human testis tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22782-1-AP targets UBR5 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18753 Product name: Recombinant human UBR5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 2450-2799 aa of BC137234 Sequence: IAHGRENGADSILDLGLVDSSEKVQQENRKRHGSSRSVVDMDLDDTDDGDDNAPLFYQPGKRGFYTPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLNRHVIKVLLGRKVNWHDFAFFDPVMYESLRQLILASQSSDADAVFSAMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLVVAEQPLHAMRKGLLDVLPKNSLEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV Predict reactive species
Full Name	ubiquitin protein ligase E3 component n-recognin 5
Calculated Molecular Weight	2799 aa, 309 kDa
Observed Molecular Weight	309 kDa
GenBank Accession Number	BC137234
Gene Symbol	UBR5
Gene ID (NCBI)	51366
RRID	AB_2879166
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95071
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for UBR5 antibody 22782-1-AP	Download protocol
IHC protocol for UBR5 antibody 22782-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
	IF	Nat Commun UBR5 promotes antiviral immunity by disengaging the transcriptional brake on RIG-I like receptors Authors - Duomeng Yang View Article
human	IF	J Gastroenterol Hepatol C2orf40 inhibits hepatocellular carcinoma through interaction with UBR5. Authors - Yue Wu View Article
human,mouse	WB,IHC,IF,CoIP	Sci China Life Sci SUB1 promotes colorectal cancer metastasis by activating NF-κB signaling via UBR5-mediated ubiquitination of UBXN1 Authors - Hao Wang View Article
human	WB,IHC,IF	Pathol Res Pract UBR5, a potential diagnostic and prognostic biomarker induces osteosarcoma progression. Authors - Mufan Li KD Validated View Article
human	WB	Drug Resist Updat RCN2 facilitates esophageal squamous cellular carcinoma metastasis and cisplatin resistance through UBR5-mediated PPP2CA ubiquitination and degradation. Authors - Mengyuan Wu KD Validated View Article
human	IF	J Transl Med Inhibition of the E3 ligase UBR5 stabilizes TERT and protects vascular organoids from oxidative stress Authors - Haijing Zhao View Article