Filter: AllWBIHC

WB analysis using 66937-1-Ig (same clone as 66937-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66937-1-Ig (same clone as 66937-1-PBS)
WB result of UBR5 antibody (66937-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBR5 transfected HeLa cells. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.
WB analysis of PC-12 using 66937-1-Ig (same clone as 66937-1-PBS)
PC-12 cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.
WB analysis using 66937-1-Ig (same clone as 66937-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66937-1-Ig (same clone as 66937-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 66937-1-Ig (same clone as 66937-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 66937-1-Ig (same clone as 66937-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.
WB analysis of Neuro-2a using 66937-1-Ig (same clone as 66937-1-PBS)
Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 66937-1-Ig (same clone as 66937-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 66937-1-Ig (same clone as 66937-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.
IHC staining of rat testis using 66937-1-Ig (same clone as 66937-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.
IHC staining of rat testis using 66937-1-Ig (same clone as 66937-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 66937-1-Ig (same clone as 66937-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 66937-1-Ig (same clone as 66937-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66937-1-Ig (same clone as 66937-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66937-1-Ig (same clone as 66937-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

UBR5 Monoclonal antibody, PBS Only

UBR5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66937-1-PBS

Clone No.2E7A6

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

DD5, EDD, EDD1, FLJ11310, hHYD, HYD, KIAA0896, Progestin induced protein, UBR5

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66937-1-PBS targets UBR5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity	Human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag18753 Product name: Recombinant human UBR5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 2450-2799 aa of BC137234 Sequence: IAHGRENGADSILDLGLVDSSEKVQQENRKRHGSSRSVVDMDLDDTDDGDDNAPLFYQPGKRGFYTPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLNRHVIKVLLGRKVNWHDFAFFDPVMYESLRQLILASQSSDADAVFSAMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLVVAEQPLHAMRKGLLDVLPKNSLEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV Predict reactive species
Full Name	ubiquitin protein ligase E3 component n-recognin 5
Calculated Molecular Weight	2799 aa, 309 kDa
Observed Molecular Weight	309 kDa
GenBank Accession Number	BC137234
Gene Symbol	UBR5
Gene ID (NCBI)	51366
RRID	AB_2882261
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	O95071
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

UBR5, also named as ubiquitin protein ligase E3 component n-reconin5, is a HECT domain-containing protein as a myocardin-binding protein. UBR5 is highly conserved in metazoans, has unique structural features, and has been implicated in regulation of DNA damage response, metabolism, transcription, and apoptosis. Hence, UBR5 is a key regulator of cell signaling relevant to broad areas of cancer biology (PMID: 26464214). The human UBR5 gene has 59 exons, encoding an approximately 10kb mRNA and >300 kDa protein with widespread expression in various cell types.